| Pregnancy and bovine somatotropin in nonlactating dairy cows: II. Endometrial gene expression related to maintenance of pregnancy. | |
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MedLine Citation:
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PMID: 15377606 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective was to evaluate the effects of pregnancy and bovine somatotropin (bST) on endometrial gene and protein expression related to maintenance of pregnancy in nonlactating dairy cows at d 17. In endometrial tissues, treatment with bST increased the steady state concentration of oxytocin receptor (OTR) mRNA; bST-treated cyclic (bST-C) cows had greater OTR mRNA than bST-treated pregnant (bST-P) cows. Estradiol receptor alpha (ERalpha) mRNA was reduced in bST-P cows compared with control P and C (no bST) cows. Western blotting revealed that pregnancy decreased the abundance of ERalpha protein, and bST stimulated an increase in ERalpha protein in C and P cows. Treatment with bST increased steady state concentrations of progesterone receptor (PR) mRNA. No differences were detected in steady state mRNA concentrations of prostaglandin H synthase-2 (PGHS-2), prostaglandin E synthase, and prostaglandin F synthase due to pregnancy or bST treatment. However, PGHS-2 protein was increased in response to pregnancy and bST treatment. Immunostaining indicated that P decreased ERalpha protein in luminal epithelium and increased PR protein in epithelial cells of the uterine glands. The PR protein response in the glands was less in bST-P cows than in P cows. In the stromal layer of the endometrium, bST decreased PR protein abundance in C and P cows. The PGHS-2 protein was localized exclusively in the luminal epithelium cells of endometrium and was increased in P cows. In conclusion, distinctly different mRNA and protein responses were detected between C and P cows related to prostaglandin biosynthesis, and bST-induced changes may potentially impact mechanisms associated with maintenance of pregnancy in nonlactating cows. |
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Authors:
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A Guzeloglu; T R Bilby; A Meikle; S Kamimura; A Kowalski; F Michel; L A MacLaren; W W Thatcher |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of dairy science Volume: 87 ISSN: 0022-0302 ISO Abbreviation: J. Dairy Sci. Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-09-20 Completed Date: 2005-01-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: United States |
Other Details:
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Languages: eng Pagination: 3268-79 Citation Subset: IM |
Affiliation:
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Department of Animal Sciences, University of Florida, Gainesville 32611-0910, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Cattle / metabolism* Dinoprost / analysis Dinoprostone / analysis Endometrium / chemistry, metabolism* Estrogen Receptor alpha / genetics Female Gene Expression / drug effects* Growth Hormone / pharmacology* Hydroxyprostaglandin Dehydrogenases / genetics Intramolecular Oxidoreductases / genetics Pregnancy Pregnancy Maintenance / genetics* Prostaglandin-Endoperoxide Synthases / analysis, genetics RNA, Messenger / analysis Receptors, Oxytocin / genetics Receptors, Progesterone / genetics |
| Chemical | |
Reg. No./Substance:
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0/Estrogen Receptor alpha; 0/RNA, Messenger; 0/Receptors, Oxytocin; 0/Receptors, Progesterone; 363-24-6/Dinoprostone; 551-11-1/Dinoprost; 89383-13-1/growth hormone, bovine; 9002-72-6/Growth Hormone; EC 1.1.1.-/Hydroxyprostaglandin Dehydrogenases; EC 1.1.1.188/prostaglandin-F synthase; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases; EC 5.3.-/Intramolecular Oxidoreductases; EC 5.3.99.3/prostaglandin-E synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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