Document Detail


Pregnancy-associated plasma protein-A and placental protein 5 in human ovarian follicular fluid.
MedLine Citation:
PMID:  2409857     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
By sensitive and specific radioimmunoassays PAPP-A and PP5 were detected in follicular aspirates obtained from women undergoing ovarian hyperstimulation for oocyte harvest prior to in vitro fertilization and embryo transfer. Follicular and pregnancy-derived PAPP-A were immunologically and physicochemically indistinguishable. Similarly, pregnancy- and nonpregnancy-derived PP5 were immunologically indistinguishable. However, in addition to the 18- and 36-K species, a larger species having a molecular size greater than 140K was found in the follicular fluid. Mean follicular PAPP-A and PP5 concentrations were 727 mIU/L and 1376 mAU/L, respectively, with no significant correlation between follicular PAPP-A, PP5, and steroid concentrations. There was, however, a significant but negative relationship with follicular volume. Preliminary in vitro studies indicated that both proteins were synthesized by granulosa cells in preparation for follicular rupture. Follicular PP5, like antithrombin III, interacted reversibly with heparin and thrombin affinity matrices, suggesting a potential biological role as a follicular anticoagulant, whereas PAPP-A, a specific and potent inhibitor of leukocyte elastase, contributes to the maintenance of proteolytic homeostasis and the protection of spermatozoa and embryo against proteolytic attack originating from the maternal leukocytes.
Authors:
M J Sinosich; D M Saunders; J G Grudzinskas
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  442     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  1985  
Date Detail:
Created Date:  1985-08-06     Completed Date:  1985-08-06     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  269-75     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Culture Media
Embryo, Mammalian / physiology
Estradiol / analysis
Female
Glycoproteins*
Humans
Ovarian Follicle / analysis*
Pregnancy
Pregnancy Proteins / analysis*,  physiology
Pregnancy-Associated Plasma Protein-A / analysis*,  physiology
Progesterone / analysis
Radioimmunoassay
Chemical
Reg. No./Substance:
0/Culture Media; 0/Glycoproteins; 0/Pregnancy Proteins; 0/tissue-factor-pathway inhibitor 2; 50-28-2/Estradiol; 57-83-0/Progesterone; EC 3.4.24.-/Pregnancy-Associated Plasma Protein-A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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