Document Detail


Pregnancy-associated growth factor. II. A T-dependent polyclonal activator of human adult peripheral blood lymphocytes (PBL).
MedLine Citation:
PMID:  2578516     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study examined the ability of pregnancy-associated growth factor (PAGF), a substance found in crude human chorionic gonadotropin (hCG), to induce plaque-forming cells (PFC) in cultured human peripheral blood lymphocytes (PBL). PAGF, 0.25 to 1 mg/ml, induced maximal PFC at 6 to 7 days as measured by the staphylococcal protein A-coupled SRBC reverse hemolytic plaque assay with a rabbit anti-human Ig antiserum. PAGF-induced PFC/culture ranged from 1800 to 39,000 with a mean of 11,524 in unfractionated PBL (N = 24), as compared to 540 to 77,840 with a mean of 17,303 for pokeweed (PWM) (N = 22). Comparison of PAGF- and PWM-induced PFC showed that both induced specific IgG, IgA, and IgM PFC. In most individuals, PAGF induced more IgM and PWM more IgG PFC. The kappa: lambda ratio was 1.5 for unstimulated PBL, and approximately 3.5 for PAGF and PWM. To see if PAGF was a T-dependent polyclonal activator of B cells, T and non-T populations were obtained by SRBC rosettes and negatively selected T4 and T8 cells by complement-mediated lysis of SRBC+(T) cells. Only the recombined subsets which included T4 cells and non-T cells supported PAGF- and PWM-induced PFC. These data indicate that PAGF, a substance derived from commercial extracts of pregnancy urine, is a T4-dependent polyclonal activator of normal human B cells.
Authors:
J A Carlino; J H Morse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  134     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-03-20     Completed Date:  1985-03-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1702-8     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
B-Lymphocytes / immunology*,  metabolism
Female
Hemolytic Plaque Technique
Humans
Immunoglobulin A / biosynthesis
Immunoglobulin Allotypes / biosynthesis
Immunoglobulin G / biosynthesis
Immunoglobulin M / biosynthesis
Lymphocyte Activation*
Male
Phenotype
Pregnancy Proteins / physiology*
T-Lymphocytes / classification,  immunology*
alpha-Macroglobulins / physiology*
Grant Support
ID/Acronym/Agency:
HD16517/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Immunoglobulin A; 0/Immunoglobulin Allotypes; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/Pregnancy Proteins; 0/alpha-Macroglobulins; 0/polyclonal B cell activator; 0/pregnancy-associated growth factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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