| Pregnancy-associated growth factor. II. A T-dependent polyclonal activator of human adult peripheral blood lymphocytes (PBL). | |
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MedLine Citation:
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PMID: 2578516 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study examined the ability of pregnancy-associated growth factor (PAGF), a substance found in crude human chorionic gonadotropin (hCG), to induce plaque-forming cells (PFC) in cultured human peripheral blood lymphocytes (PBL). PAGF, 0.25 to 1 mg/ml, induced maximal PFC at 6 to 7 days as measured by the staphylococcal protein A-coupled SRBC reverse hemolytic plaque assay with a rabbit anti-human Ig antiserum. PAGF-induced PFC/culture ranged from 1800 to 39,000 with a mean of 11,524 in unfractionated PBL (N = 24), as compared to 540 to 77,840 with a mean of 17,303 for pokeweed (PWM) (N = 22). Comparison of PAGF- and PWM-induced PFC showed that both induced specific IgG, IgA, and IgM PFC. In most individuals, PAGF induced more IgM and PWM more IgG PFC. The kappa: lambda ratio was 1.5 for unstimulated PBL, and approximately 3.5 for PAGF and PWM. To see if PAGF was a T-dependent polyclonal activator of B cells, T and non-T populations were obtained by SRBC rosettes and negatively selected T4 and T8 cells by complement-mediated lysis of SRBC+(T) cells. Only the recombined subsets which included T4 cells and non-T cells supported PAGF- and PWM-induced PFC. These data indicate that PAGF, a substance derived from commercial extracts of pregnancy urine, is a T4-dependent polyclonal activator of normal human B cells. |
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Authors:
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J A Carlino; J H Morse |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 134 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1985 Mar |
Date Detail:
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Created Date: 1985-03-20 Completed Date: 1985-03-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1702-8 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult B-Lymphocytes / immunology*, metabolism Female Hemolytic Plaque Technique Humans Immunoglobulin A / biosynthesis Immunoglobulin Allotypes / biosynthesis Immunoglobulin G / biosynthesis Immunoglobulin M / biosynthesis Lymphocyte Activation* Male Phenotype Pregnancy Proteins / physiology* T-Lymphocytes / classification, immunology* alpha-Macroglobulins / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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HD16517/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin A; 0/Immunoglobulin Allotypes; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/Pregnancy Proteins; 0/alpha-Macroglobulins; 0/polyclonal B cell activator; 0/pregnancy-associated growth factor |
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