Document Detail

Pregnancy-associated changes in oligomannose oligosaccharides of human and bovine uromodulin (Tamm-Horsfall glycoprotein).
MedLine Citation:
PMID:  2136357     Owner:  NLM     Status:  MEDLINE    
The urinary glycoprotein uromodulin (Tamm-Horsfall glycoprotein) exhibits a pregnancy-associated ability to inhibit antigen-specific T cell proliferation, and the activity is associated with a carbohydrate moiety [Muchmore and Decker (1985) Science 229:479-81; Hession et al., (1987) Science 237:1479-84; Muchmore, Shifrin and Decker (1987) J Immunol 138:2547-53]. We report here that the Man6(7)GlcNAc2-R glycopeptides derived from uromodulin inhibit antigen-specific T cell proliferation by 50% at 0.2-2 microM, and further studies, reported elsewhere, confirm that oligomannose glycopeptides from other sources are also inhibitory, with Man9GlcNAc2-R the most inhibitory of those tested [Muchmore et al., J Leukocyte Biol (in press)]. In this work, we have extended the observation of pregnancy-associated inhibitory activity to a second species, and have compared the oligomannose profile of Tamm-Horsfall glycoprotein (nonpregnant) with that of uromodulin (pregnant) derived from both human and bovine sources. Surprisingly, there was a pregnancy-associated decrease in the total content of oligomannose chains due predominantly to a reduction in Man5GlcNAc2-R and Man6GlcNAc2-R. Man7GlcNAc2-R, which did not decrease with pregnancy, comprised a significantly greater proportion of the total oligomannose chains in pregnant vs. nonpregnant samples from both species (human; 34.6% vs. 25.9%: bovine; 14.4% vs. 7.2%).
R M Smagula; H Van Halbeek; J M Decker; A V Muchmore; C E Moody; A P Sherblom
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Glycoconjugate journal     Volume:  7     ISSN:  0282-0080     ISO Abbreviation:  Glycoconj. J.     Publication Date:  1990  
Date Detail:
Created Date:  1992-09-11     Completed Date:  1992-09-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  SWEDEN    
Other Details:
Languages:  eng     Pagination:  609-24     Citation Subset:  IM    
Department of Biochemistry, University of Maine, Orono 04469.
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MeSH Terms
Carbohydrate Sequence
Cell Division
Chromatography, Gel
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Mannose / metabolism*
Molecular Sequence Data
Mucoproteins / metabolism*,  urine
Oligosaccharides / metabolism*
Pregnancy Proteins / metabolism*
T-Lymphocytes / immunology
Grant Support
Reg. No./Substance:
0/Mucoproteins; 0/Oligosaccharides; 0/Pregnancy Proteins; 0/Tamm-Horsfall protein; 31103-86-3/Mannose

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