| Pregnancy after human oocyte cryopreservation. | |
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MedLine Citation:
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PMID: 2870356 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The first successful attempt at deep freezing and thawing of the human oocyte is reported. A twin pregnancy was achieved after insemination and replacement in utero. The procedure involved reduction in size of the oocyte/cumulus-oophorus complex, the addition of the cryoprotectant dimethyl sulphoxide as a one-step procedure, slow cooling between -7 degrees C and -36 degrees C after ice nucleation, and rapid freezing to -196 degrees C before storage in liquid nitrogen. Thawing was achieved rapidly by warming in a 37 degrees C water-bath, followed by dilution of dimethyl sulphoxide as a single step. 80% of forty oocytes showed morphological survival after freezing and thawing. Thirty of these were inseminated; 83% retained their capacity to be fertilised, and 60% proceeded to cleavage division. |
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Authors:
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C Chen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Lancet Volume: 1 ISSN: 0140-6736 ISO Abbreviation: Lancet Publication Date: 1986 Apr |
Date Detail:
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Created Date: 1986-05-16 Completed Date: 1986-05-16 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 2985213R Medline TA: Lancet Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 884-6 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dimethyl Sulfoxide / pharmacology Embryo Transfer Female Freezing Humans Male Mice Mice, Inbred Strains Oocytes / growth & development, physiology* Pregnancy* Time Factors Tissue Preservation / methods* |
| Chemical | |
Reg. No./Substance:
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67-68-5/Dimethyl Sulfoxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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