Document Detail

Pregnancy after the calcium ionophore correction of pronuclei position in oocytes after intracytoplasmic sperm injection.
MedLine Citation:
PMID:  20537627     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To report a successful pregnancy after transfer of embryos derived from oocytes after calcium ionophore correction of the pronuclei localization after intracytoplasmic sperm injection (ICSI).
DESIGN: Case report.
SETTING: University hospital.
PATIENT(S): A 30-year-old patient and her 30-year-old husband, diagnosed with asthenoteratozoopermia, underwent ICSI because of three unsuccessful IUI attempts.
INTERVENTION(S): Thirteen metaphase II oocytes were injected with morphologically normal spermatozoa and immediately divided into two groups. Group 1 (n = 7) was the untreated control and in group 2 (n = 6), the oocytes were treated with 10 μM calcium ionophore solution for 20 minutes at 37°C in 6% CO(2). The fertilization was checked 18 hours later and location of pronuclei in cytoplasm was assessed. Transfer of two embryos was performed on the third day after oocyte retrieval.
MAIN OUTCOME MEASURE(S): Pregnancy after transfer of embryos after calcium ionophore correction of pronuclei localization.
RESULT(S): Fertilized zygotes from calcium ionophore-treated oocytes with physiologically normal central localization of pronuclei were chosen for ET. Clinical pregnancy was confirmed at 7 weeks of gestation.
CONCLUSION(S): Post-ICSI calcium ionophore activation of oocytes can be used for correction of the pronuclei localization, which can increase developmental rate of embryos.
Evgenia Isachenko; Vladimir Isachenko; Plamen Todorov; Vasiliy Ostashko; Rolf Kreienberg; Manfred Kaufmann; Karl Sterzik; Inka Wiegratz
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Publication Detail:
Type:  Case Reports; Journal Article     Date:  2010-05-26
Journal Detail:
Title:  Fertility and sterility     Volume:  94     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-26     Completed Date:  2011-01-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2770.e3-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Department of Obstetrics and Gynaecology, University Women Hospital Ulm, University of Ulm, Ulm, Germany.
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MeSH Terms
Calcium / metabolism*
Cell Nucleus / drug effects*
Ionophores / pharmacology*,  therapeutic use
Oocytes / drug effects*,  physiology,  ultrastructure
Sperm Injections, Intracytoplasmic*
Treatment Outcome
Zygote Intrafallopian Transfer
Reg. No./Substance:
0/Ionophores; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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