Document Detail

Pregnancy achieved with pronuclear-stage embryos that were cryopreserved and thawed twice: a case report.
MedLine Citation:
PMID:  8947819     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Our purpose was to determine if pronuclear-stage embryos (2PN) could be thawed, then frozen again with subsequent survival and cleavage after thawing. METHODS: A simplified cryopreservation protocol was used in which a slow cooling program is started at the seeding temperature of -6 degrees C in an alcohol-bath controlled-rate freezer. 1,2-Propanediol (1.5 M) was added to embryos before cooling. A fast thawing technique at room temperature was used. The cryoprotectant was removed in one step using a 1 M sucrose solution. RESULTS: Three months after refreezing, the three 2PN embryos were thawed and all three cleaved after 24 hr in culture. Following embryo transfer a pregnancy was achieved and a healthy full-term baby girl was born. CONCLUSIONS: This is the third case reported of successful pregnancies after transfer of human embryos that were frozen twice before transfer and the first case where the second freeze occurred at the pronuclear stage. This is also the first successful refreezing of human embryos using a simplified freezing and thawing technique with one-step addition and removal of cryoprotectant.
A Baker; J H Check; D Lurie; C Hourani; L M Hoover
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  13     ISSN:  1058-0468     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-02-18     Completed Date:  1997-02-18     Revised Date:  2008-03-18    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  713-5     Citation Subset:  IM    
University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden, USA.
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MeSH Terms
Embryo Transfer*
Embryo, Mammalian*
Fertilization in Vitro*
Menstrual Cycle
Pregnancy Outcome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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