Document Detail

Pregnancy achieved with pronuclear-stage embryos that were cryopreserved and thawed twice: a case report.
MedLine Citation:
PMID:  8947819     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Our purpose was to determine if pronuclear-stage embryos (2PN) could be thawed, then frozen again with subsequent survival and cleavage after thawing. METHODS: A simplified cryopreservation protocol was used in which a slow cooling program is started at the seeding temperature of -6 degrees C in an alcohol-bath controlled-rate freezer. 1,2-Propanediol (1.5 M) was added to embryos before cooling. A fast thawing technique at room temperature was used. The cryoprotectant was removed in one step using a 1 M sucrose solution. RESULTS: Three months after refreezing, the three 2PN embryos were thawed and all three cleaved after 24 hr in culture. Following embryo transfer a pregnancy was achieved and a healthy full-term baby girl was born. CONCLUSIONS: This is the third case reported of successful pregnancies after transfer of human embryos that were frozen twice before transfer and the first case where the second freeze occurred at the pronuclear stage. This is also the first successful refreezing of human embryos using a simplified freezing and thawing technique with one-step addition and removal of cryoprotectant.
A Baker; J H Check; D Lurie; C Hourani; L M Hoover
Related Documents :
3803719 - Multiple functions of segment polarity genes in drosophila.
12166639 - Vitrification of human blastocysts using the cryoloop method: successful clinical appli...
999789 - Placental agenesis, embryonal hydraemia, embryolethality and acute hypervitaminosis a i...
11495409 - Successful day 5 embryo transfer and pregnancies resulting after transport of embryos b...
14666509 - Tetraploid embryos rescue the early defects of tw5/tw5 mouse embryos.
15792629 - Metabolic changes in japanese medaka (oryzias latipes) during embryogenesis and hypoxia...
16857119 - Mid-trimester genetic amniocentesis in twin pregnancy and the risk of fetal loss.
10918099 - Serum progesterone testing to predict ectopic pregnancy in symptomatic first-trimester ...
11665299 - Two cases with dichorionic diamniotic triplet pregnancy and early cord entanglement.
Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  13     ISSN:  1058-0468     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-02-18     Completed Date:  1997-02-18     Revised Date:  2008-03-18    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  713-5     Citation Subset:  IM    
University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Embryo Transfer*
Embryo, Mammalian*
Fertilization in Vitro*
Menstrual Cycle
Pregnancy Outcome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effect of a programmed short-term stimulation protocol on the replacement of cryopreserved embryos.
Next Document:  Simultaneous program of natural-cycle in vitro fertilization and cryopreserved-thawed embryo transfe...