Document Detail

Pregnancies established from handmade cloned blastocysts reconstructed using skin fibroblasts in buffalo (Bubalus bubalis).
MedLine Citation:
PMID:  19168209     Owner:  NLM     Status:  MEDLINE    
Handmade cloning (HMC), a simple, micromanipulation-free cloning technique, has been applied for the production of cloned embryos and offspring in many livestock species. The objective of the present study was to compare the effect of donor cell type on developmental competence of HMC embryos and to explore the possibility of establishing pregnancies using these embryos in buffalo. After technical optimization of the HMC procedure for in vitro development of cloned blastocysts, various donor cells were compared for their developmental efficiency. Using buffalo fetal-, newborn-, adult fibroblasts and cumulus cells, blastocyst production rates obtained from reconstructed embryos were 24.0+/-1.8% (35/145), 33.0+/-8.0% (56/163), 21.0+/-9.3% (29/133) and 49.6+/-1.9% (77/154), respectively. Blastocyst rates were higher (P<0.05) in cumulus cell reconstructed embryos in comparison to those derived from fetal or adult fibroblasts. Pregnancy diagnosis (transrectal ultrasonography) was carried out at Day 40 of gestation. Following transfer of HMC embryos reconstructed using newborn fibroblasts 25% (2/8) buffaloes were pregnant and are at Days 201 and 94 of gestation, whereas after transfer of HMC embryos reconstructed using fetal fibroblasts, 20% (1/5) buffaloes were pregnant and are at Day 73 of gestation. In conclusion, HMC could be a simple and efficient technique for the production of cloned embryos for establishing pregnancies in buffalo.
R A Shah; A George; M K Singh; D Kumar; T Anand; M S Chauhan; R S Manik; P Palta; S K Singla
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-25
Journal Detail:
Title:  Theriogenology     Volume:  71     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-07     Completed Date:  2009-07-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1215-9     Citation Subset:  IM    
Embryo Biotechnology Lab, Animal Biotechnology Centre, National Dairy Research Institute, Karnal 132001, India.
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MeSH Terms
Blastocyst / cytology*,  physiology
Buffaloes / physiology*
Cloning, Organism / methods*
Embryonic Development / physiology
Fibroblasts / cytology*
Nuclear Transfer Techniques / veterinary
Pregnancy Outcome
Pregnancy Rate
Pregnancy, Animal*
Skin / cytology
Tissue Donors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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