Document Detail


Pregnancies and births resulting from in vitro matured oocytes fertilized with testicular spermatozoa.
MedLine Citation:
PMID:  16018244     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: In vitro maturation (IVM) of immature human oocytes is an attractive option for the treatment of infertility. Similarly, intracytoplasmic sperm injection (ICSI) followed by testicular fine needle aspiration (TEFNA) is an important treatment for primarily male-factor infertility. This report highlights the combination of these two advanced assisted reproduction techniques, namely IVM and fertilization with TEFNA-retrieved spermatozoa by ICSI to overcome both of male and female infertility problems.
METHODS: Before immature oocyte retrieval (IOR), gonadotropin stimulation was given for 3 or 5 days. Following IVM, and mature oocytes were inseminated by ICSI followed by TEFNA.
RESULTS: Four couples with five completed treatment cycles were performed, and total of 36 immature oocytes were retrieved. Following 36 to 48 h of culture, 32 (88.89%, 32/36) oocytes became mature. The mature oocytes were inseminated with TEFNA-retrieved sperm, and 18 (56.25%, 18/32) oocytes were fertilized normally following ICSI. Eleven embryos were transferred in five cycles and two pregnancies and two singleton births were achieved in two patients.
CONCLUSIONS: This result demonstrates that the successful pregnancies and live births can be established from embryos produced from in vitro matured oocytes that fertilized with testicular sperm.
Authors:
Qian Yun; Feng Ting; Juan Chen; Ling-Bo Cai; Jia-Yin Liu; Yun-Dong Mao; Wei Ding; Jia-Hao Sha
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  22     ISSN:  1058-0468     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-07-15     Completed Date:  2005-08-30     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  133-6     Citation Subset:  IM    
Affiliation:
Jiangsu Province Key Lab of Reproductive Medicine, Nanjing Medical University, P R China. qianyun@njmu.edu.cn
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MeSH Terms
Descriptor/Qualifier:
Adult
Cell Culture Techniques
Female
Fertilization in Vitro*
Gonadotropins / physiology
Humans
Male
Oocytes / growth & development*
Pregnancy
Pregnancy Outcome
Sperm Injections, Intracytoplasmic*
Spermatozoa
Testis
Chemical
Reg. No./Substance:
0/Gonadotropins
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