Document Detail


Pregnancies following artificial insemination with spermatozoa from problem stallion ejaculates processed by single layer centrifugation with androcoll-e.
MedLine Citation:
PMID:  21114793     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Some stallions produce ejaculates of low quality and/or low fertility when used for artificial insemination (AI). The purpose of these five case studies was to use Single Layer Centrifugation (SLC) to select the best spermatozoa from 'problem' ejaculates for subsequent use in AI. Sperm quality, in terms of motility, morphology and chromatin integrity, was improved in the SLC-selected samples compared to the corresponding uncentrifuged samples, with the exception of one stallion thought to have ampullary stasis. In this stallion, neither the incidence of spermatozoa with detached heads nor the proportion of damaged chromatin was decreased by SLC, in contrast to previous results. Pregnancies were obtained after using SLC-selected spermatozoa from the five stallions for AI, indicating that the spermatozoa were functional after SLC. Overall, the results suggest that SLC may be useful when preparing AI doses from some 'problem' ejaculates.
Authors:
Jm Morrell; G Mari; G Kútvölgyi; S Meurling; B Mislei; E Iacono; H Rodriguez-Martinez
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Publication Detail:
Type:  Journal Article     Date:  2010-11-30
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  46     ISSN:  1439-0531     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-07-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  642-5     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Verlag GmbH.
Affiliation:
Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, Uppsala, Sweden Dipartimento Clinico Veterinario Università di Bologna, Italy Alebäck Stud Farm, Lidköping, Sweden Flyinge AB, Flyinge, Sweden Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
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