Document Detail


Preferential release of one daughter cell during division of immobilized chinese hamster ovary cells.
MedLine Citation:
PMID:  18623191     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Chinese hamster ovary (CHO) cells were attached to tiny adhesive sites in poly-2-hydroxyethyl methacrylate(polyHEMA-) coated glass, and their divison properties were examined. The adhesive sites were produced by placing a metal mask, containing 8-mum-diameter holes arranged in a regular pattern, on top of the coated glass and exposing the sandwich to glow discharge treatment. This treatment produced an ordered array of circular cavities in the polyHEMA down to the glass. These adhesive sites were smaller in diameter than a newborn CHO cell, so that, upon division, there would theoretically be room for only one of the two new daughter cells to remain attached. It was found that individual CHO cells attached to, and grew upon, the sites, and that division normally resulted in the releas of one of the two new daughters. It is concluded that this culture technique has applications in research on the mammalian cell cycle, cell partitioning, and cellular senescence. (c) 1995 John Wiley & Sons, Inc.
Authors:
C E Helmstetter
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  45     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  2008-07-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  374-8     Citation Subset:  -    
Affiliation:
Department of Biological Sciences, Florida Institute of Technology, Melbourne, Florida 32901.
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