| Preferential reduction of the alpha-2-6-sialylation from cell surface N-glycans of human diploid fibroblastic cells by in vitro aging. | |
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MedLine Citation:
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PMID: 16897185 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human diploid fibroblastic cell line, TIG-3, has a finite life span of about 80 population doubling levels (PDL), and is used for in vitro aging studies. Young cells (PDL 23) grew to higher cell densities at a higher growth rate than aged cells (PDL 77). When the electrophoretic mobility of cells was determined, the negative surface charge of the aged cells decreased significantly when compared to that of young cells. Lectin blot analysis of membrane glycoproteins showed that the alpha-2-6-sialylation but not the alpha-2-3-sialylation of N-glycans decreases markedly in the aged cells when compared to the young cells. In support of this observation, the cDNA microarray assay and reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the gene expression of the alpha-2,6-sialyltransferase I (ST6Gal I), which transfers sialic acid to galactose residues of N-glycans, decreases in the aged cells. These results indicate that the concordant decrease of the alpha-2,6-sialylation of N-glycans with the ST6Gal I gene expression is induced in TIG-3 cells by in vitro aging. |
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Authors:
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Tomomi Tadokoro; Kiyotaka Yamamoto; Iku Kuwahara; Hirosuke Fujisawa; Masahiko Ikekita; Akiyoshi Taniguchi; Takeshi Sato; Kiyoshi Furukawa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Glycoconjugate journal Volume: 23 ISSN: 0282-0080 ISO Abbreviation: Glycoconj. J. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-08-09 Completed Date: 2007-10-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8603310 Medline TA: Glycoconj J Country: United States |
Other Details:
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Languages: eng Pagination: 443-52 Citation Subset: IM |
Affiliation:
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Department of Biosignal Research, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Aging
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physiology* Cell Line, Tumor Cell Membrane / enzymology, metabolism* Fibroblasts / metabolism* Humans N-Acetylneuraminic Acid / chemistry, metabolism* Nitrogen / chemistry, metabolism* Oxidation-Reduction Polysaccharides / chemistry, metabolism* Sialyltransferases / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Polysaccharides; 131-48-6/N-Acetylneuraminic Acid; 7727-37-9/Nitrogen; EC 2.4.99.-/Sialyltransferases; EC 2.4.99.1/beta-D-galactoside alpha 2-6-sialyltransferase |
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