| Preferential localization of exocytotic active zones in the terminals of neurite-emitting chromaffin cells. | |
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MedLine Citation:
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PMID: 9765057 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Amperometry using 2.5 microm radius carbon fiber electrodes was employed to study exocytotic catecholamine release from individual cultured bovine chromaffin cells. The secretory responses to either direct depolarization or nicotinic receptor stimulation were focal in nature in both round and neurite-emitting cells. In contrast to the random distribution of active sites found in round cells, bipolar and tripolar chromaffin cells had responsive zones preferentially located at neurite terminals as indicated by the lower probability of finding "silent" electrode positions and an increased nicotinic-receptor responsiveness when compared with the cell body. In agreement with these data we have observed a preferential deposition of dopamine-beta-hydroxylase into the neurite terminal plasmalemma after stimulation of intact cells. These observations might be of interest since the differences in the distribution of secretory "spots" between round and neurite-emitting chromaffin cells could be used to study the molecular factors determining active site localization. |
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Authors:
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L M Gutiérrez; A Gil; S Viniegra |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of cell biology Volume: 76 ISSN: 0171-9335 ISO Abbreviation: Eur. J. Cell Biol. Publication Date: 1998 Aug |
Date Detail:
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Created Date: 1998-12-11 Completed Date: 1998-12-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7906240 Medline TA: Eur J Cell Biol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 274-8 Citation Subset: IM |
Affiliation:
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Departamento de Neuroquímica, Instituto de Neurociencias and Facultad de Medicina, Universidad Miguel Hernández, Alicante, Spain. Luisguti@umh.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine
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pharmacology Animals Cattle Chromaffin Cells / cytology*, secretion, ultrastructure Dopamine beta-Hydroxylase / analysis Electrophysiology Exocytosis / physiology* Immunohistochemistry Membrane Potentials / drug effects Microelectrodes Neurites / chemistry*, drug effects, enzymology* Nicotine / pharmacology Nicotinic Agonists / pharmacology Potassium Chloride / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Nicotinic Agonists; 51-84-3/Acetylcholine; 54-11-5/Nicotine; 7447-40-7/Potassium Chloride; EC 1.14.17.1/Dopamine beta-Hydroxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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