Document Detail


Preferential localization of exocytotic active zones in the terminals of neurite-emitting chromaffin cells.
MedLine Citation:
PMID:  9765057     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Amperometry using 2.5 microm radius carbon fiber electrodes was employed to study exocytotic catecholamine release from individual cultured bovine chromaffin cells. The secretory responses to either direct depolarization or nicotinic receptor stimulation were focal in nature in both round and neurite-emitting cells. In contrast to the random distribution of active sites found in round cells, bipolar and tripolar chromaffin cells had responsive zones preferentially located at neurite terminals as indicated by the lower probability of finding "silent" electrode positions and an increased nicotinic-receptor responsiveness when compared with the cell body. In agreement with these data we have observed a preferential deposition of dopamine-beta-hydroxylase into the neurite terminal plasmalemma after stimulation of intact cells. These observations might be of interest since the differences in the distribution of secretory "spots" between round and neurite-emitting chromaffin cells could be used to study the molecular factors determining active site localization.
Authors:
L M Gutiérrez; A Gil; S Viniegra
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of cell biology     Volume:  76     ISSN:  0171-9335     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-12-11     Completed Date:  1998-12-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  274-8     Citation Subset:  IM    
Affiliation:
Departamento de Neuroquímica, Instituto de Neurociencias and Facultad de Medicina, Universidad Miguel Hernández, Alicante, Spain. Luisguti@umh.es
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / pharmacology
Animals
Cattle
Chromaffin Cells / cytology*,  secretion,  ultrastructure
Dopamine beta-Hydroxylase / analysis
Electrophysiology
Exocytosis / physiology*
Immunohistochemistry
Membrane Potentials / drug effects
Microelectrodes
Neurites / chemistry*,  drug effects,  enzymology*
Nicotine / pharmacology
Nicotinic Agonists / pharmacology
Potassium Chloride / pharmacology
Chemical
Reg. No./Substance:
0/Nicotinic Agonists; 51-84-3/Acetylcholine; 54-11-5/Nicotine; 7447-40-7/Potassium Chloride; EC 1.14.17.1/Dopamine beta-Hydroxylase

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