Document Detail


Preferential localization of the endocytic internalization machinery to hyphal tips underlies polarization of the actin cytoskeleton in Aspergillus nidulans.
MedLine Citation:
PMID:  18179595     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AbpA, SlaB and AmpA, three demonstrated components of the endocytic internalization machinery, are strongly polarized in Aspergillus nidulans hyphae, forming a ring that embraces the hyphal tip, leaving an area of exclusion at the apex. AbpA, a prototypic endocytic internalization marker, localizes to highly motile and transient (average half life, 24 +/- 5 s) peripheral punctate structures overlapping with actin patches, which also predominate in the tip. SlaB also localizes to peripheral patches, but these are markedly more abundant and cortical than those of AbpA. In contrast to its polarized distribution in hyphae, endocytic patches show random distribution during the isotropic growth phase preceding polarity establishment, but polarize as soon as a germtube primordium emerges from the swelled conidiospore. Thus, while endocytosis can occur along the hyphae, the apical predominance and the spatial organization of actin patches and of the above endocytic machinery proteins as a slightly subapical ring strongly suggests that tight spatial coupling of apical secretion and subapical compensatory endocytosis underlies hyphal growth. In agreement, the phenotype of a null slaB allele indicates that endocytosis is essential.
Authors:
Lidia Araujo-Bazán; Miguel A Peñalva; Eduardo A Espeso
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-07
Journal Detail:
Title:  Molecular microbiology     Volume:  67     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-01     Completed Date:  2008-04-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  891-905     Citation Subset:  IM    
Affiliation:
Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas CSIC, Ramiro de Maeztu, 9. Madrid 28040, Spain.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism*
Aspergillus nidulans / genetics,  metabolism*
Cell Polarity
Cytoskeleton / metabolism*
Endocytosis
Fungal Proteins / metabolism*
Genes, Fungal
Hyphae / metabolism
Chemical
Reg. No./Substance:
0/Actins; 0/Fungal Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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