| Preferential localisation of elastase in cytosol of human myeloid leukemia HL-60 cells. | |
| | |
MedLine Citation:
|
PMID: 1663366 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The subcellular distribution of the elastase in human myeloid leukemia HL-60 cells was studied in comparison with that in normal leukocytes. On differential centrifugation, most of the elastase activity of HL-60 cell lysates was recovered in the 105,000 x g supernatant, while that of human peripheral blood leukocyte lysates was recovered in the 500 x g precipitate (azurophil granule-rich fraction). Moreover, on Percoll density gradient centrifugation, the elastase activity in HL-60 cell extracts was recovered in the lightest fraction with none in the azurophil granule-rich fractions, whereas most of the activity in leukocyte extracts was recovered in the azurophil granule-rich fractions. This subcellular localization of elastase did not change when HL-60 cells differentiated into monocytes and granulocytes by induction with 12-O-tetradecanoyl phorbol-13-acetate and retinoic acid, respectively. Furthermore, on Sephadex G-75 gel filtration, the elastase activity in HL-60 cell extracts was eluted earlier than that in leukocyte extracts. The size estimation indicated that the elastase of HL-60 cells was 36-30 kDa, corresponding to the size of an elastase precursor reported. The relevance of a large form of the elastase in HL-60 cells to its subcellular localization is discussed. |
| | |
Authors:
|
G Oshima; M Yamada |
Related Documents
:
|
21499326 - Systemic treatment of focal brain injury in the rat by human umbilical cord blood cells... 21553256 - Effects of adrenalectomy and replacement therapy of corticosterone on cell proliferatio... 3522606 - Differentiation-dependent changes in the solubility of a 195-kd protein in human epider... 17763936 - Differential centrifugation in culture and differentiation of rat neural stem cells. 7691866 - Centrifugation of human spermatozoa induces sublethal damage; separation of human sperm... 354796 - Fine structural studies of the islets of langerhans in the djungarian hamster (phodopus... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biological chemistry Hoppe-Seyler Volume: 372 ISSN: 0177-3593 ISO Abbreviation: Biol. Chem. Hoppe-Seyler Publication Date: 1991 Oct |
Date Detail:
|
Created Date: 1992-02-28 Completed Date: 1992-02-28 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8503054 Medline TA: Biol Chem Hoppe Seyler Country: GERMANY |
Other Details:
|
Languages: eng Pagination: 947-53 Citation Subset: IM |
Affiliation:
|
School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Differentiation
/
physiology Cell Fractionation Centrifugation, Density Gradient Chromatography, Gel Cytosol / enzymology* Humans Leukemia, Myeloid / enzymology* Leukocytes / drug effects, enzymology Pancreatic Elastase / metabolism* Peroxidase / metabolism Tetradecanoylphorbol Acetate / pharmacology Tretinoin / pharmacology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
16561-29-8/Tetradecanoylphorbol Acetate; 302-79-4/Tretinoin; EC 1.11.1.7/Peroxidase; EC 3.4.21.36/Pancreatic Elastase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of long-chain alkylamines on arachidonate metabolism in macrophages.
Next Document: Resolution of fluent dysphasia following excision of metastatic carcinoma from the arcuate fasciculu...