Document Detail

Preferential integration of the Ad5/SV40 hybrid virus at the highly recombinogenic human chromosomal site 1p36.
MedLine Citation:
PMID:  2174396     Owner:  NLM     Status:  MEDLINE    
Human fibroblasts transformed with an adenovirus-5/simian virus 40 recombinant construct (Ad5/SV40) were analyzed to determine the chromosomal site(s) of virus integration. This was firstly done by in situ hybridization using metaphase and prometaphase chromosomes and 125I-labeled Ad5 DNA. Out of seven transformed cell lines (six of clonal origin and one uncloned), six were proven to have integrated the viral genome at the short- or the long-subtelomeric regions of autosome 1, two regions known to include chromosomal modification sites induced by acute infection with Ad12. Characterization of the integration sites was carried out by restriction analysis. Transformed cell lines with the same major chromosomal integration site were found to have the viral genome inserted in restriction fragments of different size, indicating that viral integration has occurred at different sites within a relatively small chromosomal region. Molecular studies carried out on one of the transformed cell lines (H13.1) gave an independent confirmation of the viral integration at the subterminal region of autosome 1 short arm. Nucleotide sequencing at this cellular-viral junction has shown that the virus has integrated within tandemly repeated Alu-like elements and that the cellular flanking sequences have several homologies with variable number of tandem repeats core sequences. Many possible open reading frames were identified in the DNA segment adjacent to the Alu-like elements.
M Romani; A De Ambrosis; B Alhadeff; M Purrello; Y Gluzman; M Siniscalco
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Gene     Volume:  95     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-01-16     Completed Date:  1991-01-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  231-41     Citation Subset:  IM    
Laboratory of Molecular Biology, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
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MeSH Terms
Adenoviruses, Human / genetics*
Base Sequence
Blotting, Southern
Cell Line, Transformed
Cell Transformation, Viral
Chromosomes, Human, Pair 1*
Cloning, Molecular
Genes, Viral*
Molecular Sequence Data
Nucleic Acid Conformation
Nucleic Acid Hybridization
Open Reading Frames
Recombination, Genetic*
Restriction Mapping
Simian virus 40 / genetics*
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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