Document Detail

Preferential inhibition of the synthesis of 45S ribosomal rna precursor by N-acetoxyacetylaminofluorene in rat liver epithelial cultures.
MedLine Citation:
PMID:  942886     Owner:  NLM     Status:  MEDLINE    
After exposing a line of rat liver epithelial cells to a single dose of the carcinogen N-acetoxy-2-acetylaminofluorene (N-acetoxy-AAF), a dose-dependent decrease in (3H) uridine incorporation into total cellular RNA was found. Approx. 50% inhibition occurred with 0.5 mug/ml of the compound. The kinetics of the response, the effects of actinomycin D, and the fractionation of the newly synthesized RNA by polyacrylamide gel electrophoresis indicated preferential inhibition of the synthesis of 45S ribosomal RNA precursor and relative sparing of the synthesis of heterogeneous nuclear RNA.
L A Kaplan; I B Weinstein
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Chemico-biological interactions     Volume:  12     ISSN:  0009-2797     ISO Abbreviation:  Chem. Biol. Interact.     Publication Date:  1976 Jan 
Date Detail:
Created Date:  1976-04-10     Completed Date:  1976-04-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0227276     Medline TA:  Chem Biol Interact     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  99-108     Citation Subset:  IM    
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MeSH Terms
Acetoxyacetylaminofluorene / pharmacology*
Cell Line
Dactinomycin / pharmacology
Epithelial Cells
Epithelium / drug effects,  metabolism
Fluorenes / pharmacology*
Liver / metabolism*
RNA, Ribosomal / biosynthesis*
Transcription, Genetic / drug effects*
Uridine / metabolism
Reg. No./Substance:
0/Fluorenes; 0/RNA, Ribosomal; 50-76-0/Dactinomycin; 58-96-8/Uridine; 6098-44-8/Acetoxyacetylaminofluorene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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