Document Detail


Preferential fluorescent staining of heterochromatic regions in human chromosomes 9, 15, and the Y by D 287/170.
MedLine Citation:
PMID:  10819015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The utility of a newly synthesized chemical variation of DAPI (4'-6-diamidino-2-phenyl-indole), D 287/170, for differential staining of constitutive heterochromatin in man is demonstrated. Direct staining of human chromosomes with D 287/170 results in brilliant fluorescence of the paracentromeric C-band of chromosome 9, of a proximal short-arm segment of chromosome 15, and of certain heterochromatic regions in the Y. Bright, but less conspicuous fluorescence is occasionally seen at the centromeres of other chromosomes. The staining differentiation obtained by D 287/170 is very distinct, and the intensity of the fluorescent light is unusually high. The new fluorochrome should prove particularly useful for detecting and analyzing human chromosome 9 heterochromatin at various stages of the cell cycle in normal and structurally altered chromosomes.
Authors:
W Schnedl; R Abraham; O Dann; G Geber; D Schweizer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human genetics     Volume:  59     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1981  
Date Detail:
Created Date:  2000-06-06     Completed Date:  2000-06-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  10-3     Citation Subset:  IM    
Affiliation:
Histologisch-Embryologisches Institut, Universität Wien, Austria.
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MeSH Terms
Descriptor/Qualifier:
Chromosomes, Human / ultrastructure*
Chromosomes, Human, Pair 15 / ultrastructure
Chromosomes, Human, Pair 9 / ultrastructure
Fluorescent Dyes*
Heterochromatin / ultrastructure*
Humans
Indoles*
Microscopy, Fluorescence
Staining and Labeling / methods*
Y Chromosome / ultrastructure
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Heterochromatin; 0/Indoles; 82643-00-3/D 287-170

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