| Preferential differentiation of P19 mouse embryonal carcinoma cells into smooth muscle cells. Use of retinoic acid and antisense against the central nervous system-specific POU transcription factor Brn-2. | |
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MedLine Citation:
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PMID: 8593698 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Investigation of the molecular mechanisms that control smooth muscle cell (SMC) development and differentiation is a prerequisite in understanding the regulatory mechanisms of physiological and pathological SMC-associated vascular processes. The pluripotent murine embryonal carcinoma P19 cell, whose developmental potential resembles that of early embryonic cells, can develop into cell types derived from the neuroectoderm, mesoderm, and endoderm. In the present study, we have shown a unique strategy to enhance SMC differentiation in P19 cells. Under chemical induction of high concentrations of retinoic acid (1 micromol/L), P19 cells showed optimum differentiation into SMCs. Because the P19 cells thus induced also showed differentiation into neuronal cells, a strategy to block neuronal lineage differentiation was developed using a stable transformant antisense RNA construct against Brn-2, a neuronal lineage-specific POU-domain transcription factor; thus, by specifically inhibiting neuronal differentiation, enhanced SMC differentiation by P19 cells was attained. SMC expression was confirmed by immunohistochemical staining, RNA analysis (RNase protection assay), and protein analysis (Western blot) using SMC-specific markers (eg, SM1 and calponin) and alpha-smooth muscle actin. Our results show that the pathway of SMC differentiation may provide an in vitro system useful in the investigation of SMC regulatory mechanisms (eg, transcriptional regulation) and in the further understanding of SMC development and differentiation. |
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Authors:
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T Suzuki; H S Kim; M Kurabayashi; H Hamada; H Fujii; M Aikawa; M Watanabe; N Watanabe; Y Sakomura; Y Yazaki; R Nagai |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Circulation research Volume: 78 ISSN: 0009-7330 ISO Abbreviation: Circ. Res. Publication Date: 1996 Mar |
Date Detail:
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Created Date: 1996-04-10 Completed Date: 1996-04-10 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0047103 Medline TA: Circ Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 395-404 Citation Subset: IM |
Affiliation:
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Third Department of Internal Medicine, University of Tokyo, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/S81516 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Carcinoma, Embryonal / metabolism, pathology* Cell Differentiation Fluorescent Antibody Technique, Indirect Gene Expression Homeodomain Proteins Mice Molecular Sequence Data Muscle, Smooth / cytology*, metabolism POU Domain Factors RNA, Antisense / genetics* Transcription Factors / genetics* Tretinoin / pharmacology* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Homeodomain Proteins; 0/POU Domain Factors; 0/RNA, Antisense; 0/Transcription Factors; 0/transcription factor Brn-2; 302-79-4/Tretinoin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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