| Preferential adhesion of leukocytes near bifurcations is endothelium independent. | |
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MedLine Citation:
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PMID: 20624406 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Leukocyte-endothelial interactions play central roles in many pathological conditions. However, the in vivo mechanisms responsible for nonuniform spatial distribution of adhering leukocytes to endothelial cells in microvascular networks are not clear. We used a combination of in vitro and in vivo methodologies to explain of this complex phenomenon. A mouse cremaster muscle model was used to study the spatial distribution of leukocyte-endothelial cell interaction in vivo. A PDMS-based synthetic microvascular network (SMN) device was used to study interactions of functionalized microspheres using a receptor-ligand system in a (endothelial) cell-free environment for the in vitro studies. Our in vivo and in vitro findings indicate that both leukocytes in vivo and microspheres in vitro preferentially adhere near bifurcation (within 1-2 diameters from the bifurcation). This adhesion pattern was found to be independent of the diameter of the vessels. These findings support our hypothesis that the fluidic patterns near bifurcations/junctions, and not the presence or cellular aspects of the system (e.g. cell deformation, cell signaling, heterogeneous distribution of adhesion molecules), is the main controlling factor behind the preferential adhesion patterns of leukocytes near bifurcations. |
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Authors:
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Nazanin Tousi; Bin Wang; Kapil Pant; Mohammad F Kiani; Balabhaskar Prabhakarpandian |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-07-21 |
Journal Detail:
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Title: Microvascular research Volume: 80 ISSN: 1095-9319 ISO Abbreviation: Microvasc. Res. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-15 Completed Date: 2011-02-25 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 0165035 Medline TA: Microvasc Res Country: United States |
Other Details:
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Languages: eng Pagination: 384-8 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Mechanical Engineering, Temple University, Philadelphia, PA, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Avidin / metabolism Biotin / metabolism Cell Adhesion* Endothelial Cells / physiology* Hemorheology Image Interpretation, Computer-Assisted Leukocytes / physiology* Male Mice Mice, Inbred C57BL Microcirculation Microfluidic Analytical Techniques Microscopy, Fluorescence Microvessels / cytology, physiology* Regional Blood Flow Stress, Mechanical |
| Grant Support | |
ID/Acronym/Agency:
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2R44HL076034-02/HL/NHLBI NIH HHS; R44 HL076034-02/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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1405-69-2/Avidin; 58-85-5/Biotin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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