| Predominant actions of cytosolic BSU1 and nuclear BIN2 regulate subcellular localization of BES1 in brassinosteroid signaling. | |
| | |
MedLine Citation:
|
PMID: 20387035 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
During brassinosteroid (BR) signaling in Arabidopsis, BSU1 (bri1 SUPPRESSOR1) phosphatase and BIN2 (BRASSINOSTEROID INSENSITIVE2) kinase regulate the signal intensity by determining the phosphorylation status of the transcription factors BZR1 (BRASSINAZOLE RESISTANT1) and BES1 (bri1 EMS SUPPRESSOR1). Here, we report hat BIN2 and BSU1 are nucleocytoplasmic regulators that modulate the subcellular localization of BES1, with differential activities between the nucleus and the cytosol. In our experiments, the nuclear BIN2 induced phosphorylation and nuclear export of BES1 more efficiently than cytosolic BIN2.The cytoplasmic BSU1 mediated the dephosphorylation and nuclear translocation of BES1 more efficiently than the nuclear one. BSU1 compromised the dwarf phenotype of bri1-5, a weak allele of BRI1 (BR-INSENSITIVE 1) receptor kinase, more effectively when localized in the cytosol than in the nucleus in transgenic plants. In conclusion, the predominance of cytosolic BSU1 and nuclear BIN2 might be required for the efficient subcellular localization of BES1 in BR signaling. |
| | |
Authors:
|
Hojin Ryu; Kangmin Kim; Hyunwoo Cho; Ildoo Hwang |
Related Documents
:
|
10504565 - Regulation of spinach snf1-related (snrk1) kinases by protein kinases and phosphatases ... 14673035 - Molecular genetic perspectives on cross-talk and specificity in abiotic stress signalli... 17556505 - Double mutants deficient in cytosolic and thylakoid ascorbate peroxidase reveal a compl... 12172965 - Identifying regulators of pheromone signalling in the fission yeast schizosaccharomyces... 18242725 - Erk1/2 and camkii-mediated events in memory formation: is 5ht regulation involved? 22153515 - Purple sweet potato anthocyanins attenuate hepatic lipid accumulation through activatin... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-14 |
Journal Detail:
|
Title: Molecules and cells Volume: 29 ISSN: 0219-1032 ISO Abbreviation: Mol. Cells Publication Date: 2010 Mar |
Date Detail:
|
Created Date: 2010-04-13 Completed Date: 2010-09-13 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9610936 Medline TA: Mol Cells Country: United States |
Other Details:
|
Languages: eng Pagination: 291-6 Citation Subset: IM |
Affiliation:
|
Department of Life Sciences, Pohang University of Science and Technology, Pohang, 790-894, Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Arabidopsis
/
metabolism* Arabidopsis Proteins / metabolism*, physiology* Cell Nucleus / metabolism* Cytosol / metabolism* Hydroxysteroids / metabolism* Nuclear Proteins / metabolism* Phosphoprotein Phosphatases / physiology* Plant Growth Regulators / metabolism* Protein Kinases / physiology* Signal Transduction / physiology* |
| Chemical | |
Reg. No./Substance:
|
0/Arabidopsis Proteins; 0/BES1 protein, Arabidopsis; 0/Hydroxysteroids; 0/Nuclear Proteins; 0/Plant Growth Regulators; EC 2.7.-/Protein Kinases; EC 2.7.1.-/BIN2 protein, Arabidopsis; EC 3.1.3.16/BSU1 protein, Arabidopsis; EC 3.1.3.16/Phosphoprotein Phosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Phosphorylation dependent nucleocytoplasmic shuttling of BES1 is a key regulatory event in brassinos...
Next Document: The influence of HtrA expression on the growth of Streptococcus mutans during acid stress.