Document Detail


Prednisone induces anxiety and glial cerebral changes in rats.
MedLine Citation:
PMID:  11708428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To assess whether prednisone (PDN) produces anxiety and/or cerebral glial changes in rats. METHODS: Male Wistar rats were studied and 3 groups were formed (8 rats per group). The moderate-dose group received 5 mg/kg/day PDN released from a subcutaneous implant. In the high-dose group, implants containing PDN equivalent to 60 mg/kg/day were applied. In the control group implants contained no PDN. Anxiety was assessed using an open field and elevated plus-maze devices. The number of cells and cytoplasmic transformation of astrocytes and microglia cells were assessed by immunohistochemical analyses. RESULTS: Anxiety was documented in both groups of PDN treated rats compared with controls. The magnitude of transformation of the microglia assessed by the number of intersections was significantly higher in the PDN groups than in controls in the prefrontal cortex (moderate-dose, 24.1; high-dose, 23.6; controls 18.7; p < 0.01) and striatum (moderate-dose 25.6; high-dose 26.3; controls 18.9; p < 0.01), but not in hippocampus. The number of stained microglia cells was significantly higher in the PDN treated groups in the prefrontal cortex than in controls (moderate-dose, 29.1; high-dose, 28.4; control, 17.7 cells per field; p < 0.01). Stained microglia cells were significantly more numerous striatum and hippocampus in the high-dose group compared to controls. CONCLUSION: Subacute exposure to PDN induced anxiety and reactivity of microglia. The relevance of these features for patients using PDN remains to be elucidated.
Authors:
O Gonzalez-Perez; C Ramos-Remus; J Garcia-Estrada; S Luquin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of rheumatology     Volume:  28     ISSN:  0315-162X     ISO Abbreviation:  J. Rheumatol.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-11-15     Completed Date:  2002-04-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7501984     Medline TA:  J Rheumatol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  2529-34     Citation Subset:  IM    
Affiliation:
Department of Rheumatology of the Centro Medico Nacional de Occidente, IMSS, Guadalajara, Mexico.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anxiety / chemically induced*
Astrocytes / drug effects,  metabolism,  pathology
Cerebral Cortex / drug effects*,  pathology
Corpus Striatum / drug effects,  pathology
Exploratory Behavior / drug effects
Glucocorticoids / adverse effects*
Hippocampus / drug effects,  pathology
Immunoenzyme Techniques
Male
Neuroglia / chemistry,  drug effects*,  pathology
Prednisone / adverse effects*
Prefrontal Cortex / drug effects*,  pathology
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Glucocorticoids; 53-03-2/Prednisone

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