Document Detail


Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception.
MedLine Citation:
PMID:  22692280     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Standard semen parameters are poor predictors of fertility potential. To date, apart from, paternal karyotyping sperm factors are not evaluated in recurrent pregnancy loss (RPL), only recent studies have emphasized the role of sperm factors in early embryonic development as sperm transcribes genes critical for early embryonic development. Sperm DNA integrity is useful diagnostic and prognostic marker and has clinical implications in idiopathic recurrent pregnancy loss (iRPL) following spontaneous conception. The aim of this study was to assess DNA integrity in cases experiencing iRPL following spontaneous conception.
METHODS: Semen samples from 45 patients and 20 controls were analyzed as per WHO 1999 guidelines and sperm chromatin structure assay (SCSA) was used to measure DNA fragmentation index (DFI).
RESULTS: By applying receiver operating curve (ROC) analysis, sperm DFI of approximately 26 % was found in male partner of couples experiencing iRPL.
CONCLUSIONS: Our data indicate that sperm from men with a history of iRPL have a higher percentage of DNA damage as compared to control group, and this can explain pregnancy loss in these patients. Men with higher DFI are infertile whereas men with lower DFI (26 %) are able to conceive but experience recurrent pregnancy loss. Thus it is important to evaluate sperm DFI in couples experiencing iRPL to understand exact aetiology of RPL and determine prognosis and management.
Authors:
Kishlay Kumar; Deepika Deka; Amar Singh; D K Mitra; B R Vanitha; Rima Dada
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-13
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  29     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-10-11     Completed Date:  2013-03-11     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  861-7     Citation Subset:  IM    
Affiliation:
Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Sciences, New Delhi 29, India. kishlaykumar@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Case-Control Studies
Chromatin / genetics
DNA Fragmentation*
Embryo Loss / genetics*,  pathology
Female
Fertilization / genetics*
Humans
Infertility, Male
Karyotyping / methods
Male
Middle Aged
Predictive Value of Tests
Pregnancy
Pregnancy Outcome
Prognosis
ROC Curve
Semen / cytology
Semen Analysis / methods
Sensitivity and Specificity
Spermatozoa / pathology*
Chemical
Reg. No./Substance:
0/Chromatin
Comments/Corrections

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