Document Detail


Predictive utility of the attached segment in the quality control of a cord blood graft.
MedLine Citation:
PMID:  15812389     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The limited number of progenitor stem cells in umbilical cord blood (UCB) enforces the optimization and strict control of all the procedures involved in its therapeutic use--ie, collection, processing, cryopreservation, thawing, and transportation--to ensure graft potency at transplantation. For this reason, international UCB standards recommend storage of a cell sample attached to the UCB unit as a quantitative and functional control of the unit selected for transplantation. To validate the use of the sample attached to the UCB unit as a quality-control tool for the final product, UCB units (n = 20) stored in liquid nitrogen with the Bioarchive system were analyzed. The UCB units and their attached segments were thawed, and the number and viability of total nucleated cells, mononucleated cells, CD45 + cells, and CD34+ cells were determined, as were colony-forming cell counts. There was no significant difference between UCB units and segments for any of the parameters assessed. Additionally, the linear correlation coefficient (R2) in these paired samples was 0.85 and 0.78 for CD34+ cells and colony-forming cells, respectively. In conclusion, the cell sample in the tube segment physically linked to the transplant UCB bag predicts the total cell content and functionality of the unit and may serve as a source for final quality control of the UCB unit before transplantation.
Authors:
Luciano Rodríguez; Joan García; Sergi Querol
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation     Volume:  11     ISSN:  1083-8791     ISO Abbreviation:  Biol. Blood Marrow Transplant.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-06     Completed Date:  2005-08-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9600628     Medline TA:  Biol Blood Marrow Transplant     Country:  United States    
Other Details:
Languages:  eng     Pagination:  247-51     Citation Subset:  IM    
Affiliation:
Barcelona Cord Blood Bank, Transfusion Centre and Tissue Bank, Hospital Duran i Reynals, Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Blood Cell Count
Cell Survival
Cord Blood Stem Cell Transplantation / standards
Cryopreservation* / standards
Fetal Blood* / cytology
Humans
Public Health Practice / standards
Quality of Health Care* / standards,  trends
Stem Cells* / cytology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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