Document Detail


Predictive observation-based endpoint criteria for mice receiving total body irradiation.
MedLine Citation:
PMID:  24209966     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Total body irradiation of mice is a commonly used research technique; however, humane endpoints have not been clearly identified. This situation has led to the inconsistent use of various endpoints, including death. To address this issue, we refined a cageside observation-based scoring system specifically for mice receiving total body irradiated. Male and female C57BL/6 mice (age, 8 wk) received 1 of 3 doses of radiation from 1 of 2 different radiation sources and were observed for progression of clinical signs. All mice were scored individually by using cageside observations of their body posture (score, 0 to 3), eye appearance (0 to 3), and activity level (0 to 3). Retrospective analysis of the observation score data indicated that death could be predicted accurately with total scores of 7 or greater, and observation scores were consistent between observers. This scoring system can be used to increase the consistent use of endpoint criteria in total body murine irradiation studies and ultimately to improve animal welfare.
Authors:
Elizabeth A Nunamaker; Robert J Anderson; James E Artwohl; Alexander V Lyubimov; Jeffrey D Fortman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Comparative medicine     Volume:  63     ISSN:  1532-0820     ISO Abbreviation:  Comp. Med.     Publication Date:  2013  
Date Detail:
Created Date:  2013-11-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100900466     Medline TA:  Comp Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  313-22     Citation Subset:  IM    
Affiliation:
Biologic Resources Laboratory, College of Medicine, University of Illinois at Chicago, Chicago, Illinois, USA; MED Institute, West Lafayette, Indiana, USA. lizzynun@gmail.com.
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