Document Detail


Prediction of tolerance in children with IgE mediated cow's milk allergy by microarray profiling and chemometric approach.
MedLine Citation:
PMID:  22580759     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The sera of a retrospective cohort (n=41) composed of children with well characterized cow's milk allergy collected from multiple visits were analyzed using a protein microarray system measuring four classes of immunoglobulins. The frequency of the visits, age and gender distribution reflected real situation faced by the clinicians at a pediatric reference center for food allergy in São Paulo, Brazil. The profiling array results have shown that total IgG and IgA share similar specificity whilst IgM and in particular IgE are distantly related. The correlation of specificity of IgE and IgA is variable amongst the patients and this relationship cannot be used to predict atopy or the onset of tolerance to milk. The array profiling technique has corroborated the clinical selection criteria for this cohort albeit it clearly suggested that 4 out of the 41 patients might have allergies other than milk origin. There was also a good correlation between the array data and ImmunoCAP results, casein in particular. By using qualitative and quantitative multivariate analysis routines it was possible to produce validated statistical models to predict with reasonable accuracy the onset of tolerance to milk proteins. If expanded to larger study groups, the array profiling in combination with the multivariate techniques show potential to improve the prognostic of milk allergic patients.
Authors:
F Wulfert; G Sanyasi; L Tongen; L A Watanabe; X Wang; N K Renault; F H Falcone; C M A Jacob; M J C Alcocer
Related Documents :
15704239 - Comparison of the 2,2'-azinobis-3-ethylbenzotiazo-line-6-sulfonic acid (abts) and ferri...
17867639 - Development of an enzyme-linked immunosorbent assay to detect an immunomodulatory alpha...
14509339 - Study of the polyphenol content of red and white grape varieties by liquid chromatograp...
16492529 - Anticariogenic activity of macelignan isolated from myristica fragrans (nutmeg) against...
16616759 - Influence of the extraction methodology on the analysis of polycyclic aromatic hydrocar...
20717869 - Procyanidins from apples (malus pumila mill.) extend the lifespan of caenorhabditis ele...
16376969 - Estimation of 1999-2004 dietary daily intake of pcdds, pcdfs and dioxin-like pcbs by a ...
22504779 - Neural correlates of stress and favorite-food cue exposure in adolescents: a functional...
7112889 - The hygienic treatment of turkey eggs by dipping in an antibiotic and disinfectant solu...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-8
Journal Detail:
Title:  Journal of immunological methods     Volume:  -     ISSN:  1872-7905     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
Department of Biosciences, Sheffield Hallam University, City Campus, Sheffield, SW1 1WB, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Differential effects of mechanical strain on osteoclastogenesis and osteoclast-related g...
Next Document:  Autoantibodies directed against labile epitopes on cell surface proteins in autoimmune disease patie...