Document Detail

Prediction of rabbit caecal fermentation characteristics from faeces by in vitro gas production technique: roughages.
MedLine Citation:
PMID:  18477306     Owner:  NLM     Status:  MEDLINE    
To find equations able to estimate the fermentation characteristics of the caecum from that of faeces, caecal content and faeces of 10 hybrid Hyla rabbits were used as inocula for an in vitro gas production trial. About 1 g of 12 roughages, 11 hays (ryegrass, alfalfa, sulla, oat, vetch, sulla-lolium, vetch-oat, sulla-oat, clover, ryegrass-clover, sulla-vetch-oat) and a wheat straw, was weighed, in triplicate per inoculum, in 120-ml flasks; 75 ml of anaerobic medium and 4 ml of reducing solution were added and the flasks were placed at 39 degrees C. Caecal content and faeces were diluted respectively 1:2 (CI) and 1:8 (FI) with anaerobic medium and were introduced into their respective flasks (10 ml). Gas production was recorded 20 times at 2-24 h intervals throughout fermentation (120 h). The fermentation characteristics (i.e. degraded organic matter, OMd; potential gas production, A; maximum fermentation rate, R(max); volatile fatty acid, VFA; ammonia, NH(3)) were studied by inocula and substrates. The two inocula did not differ in OMd but CI produced significantly higher gas (A, 213.1 vs. 199.4 ml/g, respectively, for CI and FI, p < 0.01) in less time (R(max), 3.08 vs. 2.24 ml/h, respectively, for CI and FI, p < 0.01). CI also produced higher levels of total VFA (57.86 vs. 46.70 mmol/g OM, respectively, for CI and FI, p < 0.01) and showed a higher branched chain proportion (0.023 vs. 0.018, respectively, for CI and FI, p < 0.01). For some parameters (as OMd pH and propionate) the equations for the estimation of caecal fermentation characteristics from that of faeces were accurate (R(2) > 0.8828) and reliable (CV < 10.78%) suggesting that faeces can be successfully used for the estimation of these parameters.
F Bovera; S Calabrò; M I Cutrignelli; F Infascelli; G Piccolo; S Nizza; R Tudisco; A Nizza
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of animal physiology and animal nutrition     Volume:  92     ISSN:  1439-0396     ISO Abbreviation:  J Anim Physiol Anim Nutr (Berl)     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-14     Completed Date:  2008-07-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101126979     Medline TA:  J Anim Physiol Anim Nutr (Berl)     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  260-71     Citation Subset:  IM    
Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Faculty of Veterinary Medicine, University of Napoli Federico II, Naples, Italy.
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MeSH Terms
Ammonia / analysis
Animal Feed
Animal Nutritional Physiological Phenomena
Bacteria / metabolism*
Cecum / metabolism*,  microbiology
Dietary Fiber / metabolism*,  microbiology
Fatty Acids, Volatile / analysis
Feces / chemistry*
Rabbits / metabolism*
Reg. No./Substance:
0/Fatty Acids, Volatile; 0/Gases; 7664-41-7/Ammonia

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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