Document Detail


Prediction of oocyte developmental competence in ovine using glucose-6-phosphate dehydrogenase (G6PDH) activity determined at retrieval time.
MedLine Citation:
PMID:  21870182     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To determine whether G6PDH-activity measured by Brilliant Cresyl Blue known as BCB dye, predicts developmental competence within cohorts of ovine oocytes.
METHODS: Ovine oocytes were exposed to BCB staining and categorized into two groups: BCB+ (blue cytoplasm, low G6PDH-activity) and BCB- (colorless cytoplasm, high G6PDH-activity). After maturation in vitro, oocytes were subjected to fertilization followed by in vitro embryo culture.
RESULTS: We observed a significant difference in oocyte diameter considering BCB+ and BCB- oocytes. BCB+ and Control groups showed significantly higher maturation rates compared to BCB- group. There were significantly more cleaved embryos in BCB+ and control groups than in BCB- group. Blastocyst rate was significantly higher for BCB+ group compared to control and BCB- groups with control group being significantly higher than BCB- group.
CONCLUSION: G6PDH-activity is a strong predictive marker of oocyte competence and may be useful in identifying oocytes with a good prognosis for further develop.
Authors:
Abdollah Mohammadi-Sangcheshmeh; Masoud Soleimani; Hamid Deldar; Mohammad Salehi; Sara Soudi; Seyed Mahmoud Hashemi; Karl Schellander; Michael Hoelker
Publication Detail:
Type:  Journal Article     Date:  2011-08-26
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  29     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-02     Completed Date:  2012-07-31     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  153-8     Citation Subset:  IM    
Affiliation:
Department of Transgenic Animal Science, Stem Cell Technology Research Center, Tehran, Iran. a.mohammadis@stemcellstech.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / cytology
Embryo Transfer / methods
Female
Fertilization in Vitro / methods
Glucosephosphate Dehydrogenase / metabolism*
In Vitro Oocyte Maturation Techniques*
Oocytes / enzymology*,  growth & development*
Predictive Value of Tests
Sheep / embryology
Chemical
Reg. No./Substance:
EC 1.1.1.49/Glucosephosphate Dehydrogenase
Comments/Corrections

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