| Prediction of in-vitro developmental competence of early cleavage-stage mouse embryos with compact time-lapse equipment. | |
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MedLine Citation:
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PMID: 20089456 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Single blastocyst transfer is regarded as an efficient way to achieve high pregnancy rates and to avoid multiple pregnancies. Risk of cancellation of transfer due to a lack of available embryos may be reduced by early prediction of blastocyst development. Time-lapse investigation of mouse embryos shows that the time of the first and second cleavage (to the 2- and 3-cell stages, respectively) has a strong predictive value for further development in vitro, while cleavage from the 3-cell to the 4-cell stage has no predictive value. In humans, embryo fragmentation during preimplantation development has been associated with lower pregnancy rates and a higher incidence of developmental abnormalities. Analysis of time-lapse records shows that most fragmentation is reversible in the mouse and is resorbed in an average of 9 h. Daily or bi-daily microscopic checks of embryo development, applied routinely in human IVF laboratories, would fail to detect 36 or 72% of these fragmentations, respectively. Fragmentation occurring in a defined time frame has a strong predictive value for in-vitro embryo development. The practical compact system used in the present trial, based on the 'one camera per patient' principle, has eliminated the usual disadvantages of time-lapse investigations and is applicable for the routine follow-up of in-vitro embryo development. |
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Authors:
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Csaba Pribenszky; Eszter Losonczi; Miklós Molnár; Zsolt Lang; Szabolcs Mátyás; Klára Rajczy; Katalin Molnár; Péter Kovács; Péter Nagy; Jason Conceicao; Gábor Vajta |
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Publication Detail:
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Type: Journal Article Date: 2010-01-25 |
Journal Detail:
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Title: Reproductive biomedicine online Volume: 20 ISSN: 1472-6491 ISO Abbreviation: Reprod. Biomed. Online Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-26 Completed Date: 2010-12-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101122473 Medline TA: Reprod Biomed Online Country: England |
Other Details:
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Languages: eng Pagination: 371-9 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Animal Breeding and Genetics, Faculty of Veterinary Science, Szent István University, István u. 2, Budapest 1078, Hungary. pribenszky.csaba@aotk.szie.hu |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Embryo Culture Techniques / instrumentation Embryo Transfer / methods* Embryonic Development* Female Mice Photography / instrumentation* Predictive Value of Tests Pregnancy |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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