Document Detail


Prediction of in-vitro developmental competence of early cleavage-stage mouse embryos with compact time-lapse equipment.
MedLine Citation:
PMID:  20089456     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Single blastocyst transfer is regarded as an efficient way to achieve high pregnancy rates and to avoid multiple pregnancies. Risk of cancellation of transfer due to a lack of available embryos may be reduced by early prediction of blastocyst development. Time-lapse investigation of mouse embryos shows that the time of the first and second cleavage (to the 2- and 3-cell stages, respectively) has a strong predictive value for further development in vitro, while cleavage from the 3-cell to the 4-cell stage has no predictive value. In humans, embryo fragmentation during preimplantation development has been associated with lower pregnancy rates and a higher incidence of developmental abnormalities. Analysis of time-lapse records shows that most fragmentation is reversible in the mouse and is resorbed in an average of 9 h. Daily or bi-daily microscopic checks of embryo development, applied routinely in human IVF laboratories, would fail to detect 36 or 72% of these fragmentations, respectively. Fragmentation occurring in a defined time frame has a strong predictive value for in-vitro embryo development. The practical compact system used in the present trial, based on the 'one camera per patient' principle, has eliminated the usual disadvantages of time-lapse investigations and is applicable for the routine follow-up of in-vitro embryo development.
Authors:
Csaba Pribenszky; Eszter Losonczi; Miklós Molnár; Zsolt Lang; Szabolcs Mátyás; Klára Rajczy; Katalin Molnár; Péter Kovács; Péter Nagy; Jason Conceicao; Gábor Vajta
Related Documents :
17118586 - Synchronization and superovulation of mature cycling gilts for the collection of pronuc...
2815116 - Mutagenicity testing of doxylamine succinate, an antinauseant drug.
16726476 - Superovulation, collection and transfer of embryos and demi-embryos from boran(bos indi...
18971426 - Disruption of mitochondrial malate-aspartate shuttle activity in mouse blastocysts impa...
19566556 - Pregnancy outcome in the setting of extremely low first trimester papp-a levels.
24262136 - Do placental histologic findings of chorion-decidual hemorrhage or inflammation in spon...
Publication Detail:
Type:  Journal Article     Date:  2010-01-25
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  20     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-26     Completed Date:  2010-12-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  371-9     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
Affiliation:
Department of Animal Breeding and Genetics, Faculty of Veterinary Science, Szent István University, István u. 2, Budapest 1078, Hungary. pribenszky.csaba@aotk.szie.hu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Embryo Culture Techniques / instrumentation
Embryo Transfer / methods*
Embryonic Development*
Female
Mice
Photography / instrumentation*
Predictive Value of Tests
Pregnancy

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of N-acetyl-cysteine after ovarian drilling in clomiphene citrate-resistant PCOS women: a pil...
Next Document:  SCOTBLOOD 2009: The Quest for Understanding vCJD; Claudia's Trachea Implantation; Transfusion Trigge...