Document Detail

Prediction of in-vitro developmental competence of early cleavage-stage mouse embryos with compact time-lapse equipment.
MedLine Citation:
PMID:  20089456     Owner:  NLM     Status:  MEDLINE    
Single blastocyst transfer is regarded as an efficient way to achieve high pregnancy rates and to avoid multiple pregnancies. Risk of cancellation of transfer due to a lack of available embryos may be reduced by early prediction of blastocyst development. Time-lapse investigation of mouse embryos shows that the time of the first and second cleavage (to the 2- and 3-cell stages, respectively) has a strong predictive value for further development in vitro, while cleavage from the 3-cell to the 4-cell stage has no predictive value. In humans, embryo fragmentation during preimplantation development has been associated with lower pregnancy rates and a higher incidence of developmental abnormalities. Analysis of time-lapse records shows that most fragmentation is reversible in the mouse and is resorbed in an average of 9 h. Daily or bi-daily microscopic checks of embryo development, applied routinely in human IVF laboratories, would fail to detect 36 or 72% of these fragmentations, respectively. Fragmentation occurring in a defined time frame has a strong predictive value for in-vitro embryo development. The practical compact system used in the present trial, based on the 'one camera per patient' principle, has eliminated the usual disadvantages of time-lapse investigations and is applicable for the routine follow-up of in-vitro embryo development.
Csaba Pribenszky; Eszter Losonczi; Miklós Molnár; Zsolt Lang; Szabolcs Mátyás; Klára Rajczy; Katalin Molnár; Péter Kovács; Péter Nagy; Jason Conceicao; Gábor Vajta
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Publication Detail:
Type:  Journal Article     Date:  2010-01-25
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  20     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-26     Completed Date:  2010-12-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  371-9     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
Department of Animal Breeding and Genetics, Faculty of Veterinary Science, Szent István University, István u. 2, Budapest 1078, Hungary.
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MeSH Terms
Embryo Culture Techniques / instrumentation
Embryo Transfer / methods*
Embryonic Development*
Photography / instrumentation*
Predictive Value of Tests

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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