Document Detail


Prediction of in-vitro developmental competence of early cleavage-stage mouse embryos with compact time-lapse equipment.
MedLine Citation:
PMID:  20089456     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Single blastocyst transfer is regarded as an efficient way to achieve high pregnancy rates and to avoid multiple pregnancies. Risk of cancellation of transfer due to a lack of available embryos may be reduced by early prediction of blastocyst development. Time-lapse investigation of mouse embryos shows that the time of the first and second cleavage (to the 2- and 3-cell stages, respectively) has a strong predictive value for further development in vitro, while cleavage from the 3-cell to the 4-cell stage has no predictive value. In humans, embryo fragmentation during preimplantation development has been associated with lower pregnancy rates and a higher incidence of developmental abnormalities. Analysis of time-lapse records shows that most fragmentation is reversible in the mouse and is resorbed in an average of 9 h. Daily or bi-daily microscopic checks of embryo development, applied routinely in human IVF laboratories, would fail to detect 36 or 72% of these fragmentations, respectively. Fragmentation occurring in a defined time frame has a strong predictive value for in-vitro embryo development. The practical compact system used in the present trial, based on the 'one camera per patient' principle, has eliminated the usual disadvantages of time-lapse investigations and is applicable for the routine follow-up of in-vitro embryo development.
Authors:
Csaba Pribenszky; Eszter Losonczi; Miklós Molnár; Zsolt Lang; Szabolcs Mátyás; Klára Rajczy; Katalin Molnár; Péter Kovács; Péter Nagy; Jason Conceicao; Gábor Vajta
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Publication Detail:
Type:  Journal Article     Date:  2010-01-25
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  20     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-26     Completed Date:  2010-12-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  371-9     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
Affiliation:
Department of Animal Breeding and Genetics, Faculty of Veterinary Science, Szent István University, István u. 2, Budapest 1078, Hungary. pribenszky.csaba@aotk.szie.hu
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MeSH Terms
Descriptor/Qualifier:
Animals
Embryo Culture Techniques / instrumentation
Embryo Transfer / methods*
Embryonic Development*
Female
Mice
Photography / instrumentation*
Predictive Value of Tests
Pregnancy

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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