Document Detail


Prediction of analyte retention for ion chromatography separations performed using elution profiles comprising multiple isocratic and gradient steps.
MedLine Citation:
PMID:  18327920     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
This study addresses the simulation of ion chromatographic (IC) separations performed under conditions where the elution profile consists of a sequence of isocratic and gradient elution steps (referred to as "complex elution profiles"). First, models for prediction of retention under gradient elution conditions in IC were evaluated using an extensive database of gradient elution retention data. It is shown that one such model is preferred on the basis that it can be used to predict gradient retention times on the basis of isocratic input data. A method is then proposed for using this model for complex elution profiles whereby each step of the elution profile is treated separately and analyte movement through the column is mapped. An empirically based algorithm for predicting peak width under complex elution conditions is also proposed. Evaluation of the suggested approaches was undertaken on a set of 24 analyte anions and 13 analyte cations on 5 different Dionex columns using a range of 5-step complex elution profiles that gave R2 values for correlations between predicted and observed retention times of 0.987 for anions and 0.997 for cations. The simulation of separations of anions and cations using a 3-step complex elution profile is demonstrated, with good correlation between observed and predicted chromatograms. The proposed approach is useful for the rapid development of separations when complex elution profiles are used in IC.
Authors:
Robert A Shellie; Boon K Ng; Greg W Dicinoski; Samuel D H Poynter; John W O'Reilly; Christopher A Pohl; Paul R Haddad
Publication Detail:
Type:  Journal Article     Date:  2008-03-08
Journal Detail:
Title:  Analytical chemistry     Volume:  80     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-31     Completed Date:  2008-06-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2474-82     Citation Subset:  -    
Affiliation:
Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, University of Tasmania, Private Bag 75, Hobart 7001 Australia.
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