Document Detail


Prediction of Michaelis-Menten Constant of beta-Glucosidases using Nitrophenyl-beta-D-glucopyranoside as Substrate.
MedLine Citation:
PMID:  21592074     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In this study, we attempted to use the neural network to model a quantitative structure-K(m) (Michaelis-Menten constant) relationship for beta-glucosidase, which is an important enzyme to cut the beta-bond linkage in glucose while K(m) is a very important parameter in enzymatic reactions. Eight feedforward backpropagation neural networks with different layers and neurons were applied for the development of predictive model, and twenty-five different features of amino acids were chosen as predictors one by one. The results show that the 20-1 feedforward backpropagation neural network can serve as a predictive model while the normalized polarizability index as well as the amino-acid distribution probability can serve as the predictors. This study threw lights on the possibility of predicting the K(m) in beta-glucosidases based on their amino-acid features.
Authors:
Shaomin Yan; Guang Wu
Related Documents :
12371474 - Photoreduction of the chloropropionic acid of carfentrazone-ethyl in sodium sulfide [co...
163684 - Blastomogenic activity of p-hydroxyphenyllactic acid in mice.
19903444 - Quantitation of folates and their catabolites in blood plasma, erythrocytes, and urine ...
17117344 - Inhibition of folic acid uptake by catechins and tea extracts in caco-2 cells.
22975914 - Elimination of influence by interference substance on amperometric enzyme biosensor res...
17516074 - Regulation of fat/cd36 mrna gene expression by long chain fatty acids in the differenti...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-19
Journal Detail:
Title:  Protein and peptide letters     Volume:  -     ISSN:  1875-5305     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9441434     Medline TA:  Protein Pept Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
State Key Laboratory of Non-food Biomass Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi, 530007, China. hongguanglishibahao@yahoo.com.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Mapping Fragmental Drug-likeness in the MoStBioDat Environment: Intramolecular Hydrogen Bonding Moti...
Next Document:  Analysis Of Rate-Limiting Long-Range Contacts In The Folding Rate Of Three-State And Two-State Prote...