| Predicting the survival rate of mouse embryonic stem cells cryopreserved in alginate beads. | |
| | |
MedLine Citation:
|
PMID: 22292208 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Stem cell cryopreservation in three-dimensional (3D) scaffolds may offer better protection to cells leading to higher survival rates. However, it introduces heterogeneity in cryoprotective agent (CPA) concentrations, durations of exposure to CPA, and freezing and thawing rate within constructs. This paper applies a mathematical model which couples the mass transport of dimethyl sulphoxide (DMSO) in a cell-seeded spherical construct and cell membrane transport into mouse embryonic stem cells (mESCs) to predict overall cell survival rate (CSR) based on CPA equilibrium exposure times (t(E)) and concentrations. The effect of freeze-concentration is also considered. To enable such a prediction, a contour plot was constructed using experimental data obtained in cryopreservation of cell suspensions with DMSO at a cooling rate of 1 degrees C/min. Thereafter, the diffusion in the alginate bead and the membrane transport of CPA was numerically simulated. Results were mapped onto the survival rate contours yielding 'predicted' CSR. The effects of loading time, hindrance, construct radius, and CPA concentration on predicted CSR were examined. From these results, an operation window with upper and lower t(E) of 12-19 min (for 0.6 mm radius beads and 1.4 M DMSO) yielded an overall viability of 60 per cent. The model predictions and the best experimental cryopreservation results with encapsulated mESCs were in agreement. Hence, optimization based on post-thaw CSR can accelerate the identification of cryopreservation protocols and parameters for maximizing cell survival. |
| | |
Authors:
|
S Sambu; X Xu; H Ye; Z F Cui |
Related Documents
:
|
20490938 - Micro/nano-fabrication technologies for cell biology. 21556798 - Histone deacetylase inhibitor augments anti-tumor effect of gemcitabine and pegylated i... 21526718 - Rnai silenced dd-grp94 (dictyostelium discoideum glucose-regulated protein 94 kda) cell... 22510768 - Pattern of deposition of cell wall polysaccharides and transcript abundance of related ... 15698668 - In vivo labeling of neuroblasts in the subventricular zone of rats. 16612388 - The reversibility of mitotic exit in vertebrate cells. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine Volume: 225 ISSN: 0954-4119 ISO Abbreviation: Proc Inst Mech Eng H Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2012-02-01 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8908934 Medline TA: Proc Inst Mech Eng H Country: England |
Other Details:
|
Languages: eng Pagination: 1092-107 Citation Subset: IM |
Affiliation:
|
Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Grant Support | |
ID/Acronym/Agency:
|
//Biotechnology and Biological Sciences Research Council |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evaluating the ability of posterior elements to support new instrumentation for spinal fusion.
Next Document: Simultaneous repair of two large cranial defects using rapid prototyping and custom computer-designe...