Document Detail

Predicting in vitro tissue culture growth for cytogenetic evaluation of stillborn fetuses.
MedLine Citation:
PMID:  3888714     Owner:  NLM     Status:  MEDLINE    
A prospective study was undertaken of 131 perinatal deaths to determine whether gestational age, body weight, maceration degree and autopsy interval influenced successful in vitro tissue culture for cytogenetic evaluation. Perinatal populations were categorized as neonatal death (NND), fresh stillbirth (FSB), or graded as macerated stillbirth (MAC-0, MAC-1, MAC-2, MAC-3). Metaphase production by at least 15 cells separated 'growth' from 'no growth' categories after sampling liver, kidney and spleen. Body weight and degree of maceration were predictive of successful 'growth', while gestational age and autopsy interval were not. Body weight was significant in separating 'growth' from 'no growth' in NND (P = 0.05), FSB, MAC-0 and MAC-1 (P = 0.01). Growth probabilities were 0.78 (NND), 0.57 (FSB), 0.49 (MAC-0), 0.38 (MAC-1) and zero for MAC-2 and MAC-3. We conclude that (a) tissues from MAC-2 and MAC-3 fetuses do not grow and thus need not be sampled at autopsy, (b) maceration degree and body weight can be used to predict the growth probability in the other categories, (c) tissue samples can be taken during daylight hours, since autopsy interval does not influence successful growth provided the fetus is refrigerated at 4 degrees C, (d) all of the above conclusions have cost-efficiency implications for cytogenetic laboratories.
T A Macpherson; K L Garver; J H Turner; G R Diggans; S G Marchese; G C Poole
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of obstetrics, gynecology, and reproductive biology     Volume:  19     ISSN:  0301-2115     ISO Abbreviation:  Eur. J. Obstet. Gynecol. Reprod. Biol.     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-06-26     Completed Date:  1985-06-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0375672     Medline TA:  Eur J Obstet Gynecol Reprod Biol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  167-74     Citation Subset:  IM    
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MeSH Terms
Body Weight
Cell Survival
Culture Techniques
Fetal Death / physiopathology*
Gestational Age
Histological Techniques
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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