| Prediabetic nephropathy as an early consequence of the high-calorie/high-fat diet: relation to oxidative stress. | |
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MedLine Citation:
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PMID: 22234462 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study evaluated early renal functional, structural, and biochemical changes in high-calorie/high-fat diet fed mice, a model of prediabetes and alimentary obesity. Male C57BL6/J mice were fed normal (11 kcal% fat) or high-fat (58 kcal% fat) diets for 16 wk. Renal changes were evaluated by histochemistry and immunohistochemistry, Western blot analysis, ELISA, enzymatic assays, and chemiluminometry. High-fat diet consumption led to increased body and kidney weights, impaired glucose tolerance, hyperinsulinemia, polyuria, a 2.7-fold increase in 24-h urinary albumin excretion, 20% increase in renal glomerular volume, 18% increase in renal collagen deposition, and 8% drop of glomerular podocytes. It also resulted in a 5.3-fold increase in urinary 8-isoprostane excretion and a 38% increase in renal cortex 4-hydroxynonenal adduct accumulation. 4-hydroxynonenal adduct level and immunoreactivity or Sirtuin 1 expression in renal medulla were not affected. Studies of potential mechanisms of the high-fat diet induced renal cortex oxidative injury revealed that whereas nicotinamide adenine dinucleotide phosphate reduced form oxidase activity only tended to increase, 12/15-lipoxygenase was significantly up-regulated, with approximately 12% increase in the enzyme protein expression and approximately 2-fold accumulation of 12(S)-hydroxyeicosatetraenoic acid, a marker of 12/15-lipoxygenase activity. Accumulation of periodic acid-Schiff -positive material, concentrations of TGF-β, sorbitol pathway intermediates, and expression of nephrin, CAAT/enhancer-binding protein homologous protein, phosphoeukaryotic initiation factor-α, and total eukaryotic initiation factor-α in the renal cortex were indistinguishable between experimental groups. Vascular endothelial growth factor concentrations were reduced in high-fat diet fed mice. In conclusion, systemic and renal cortex oxidative stress associated with 12/15-lipoxygenase overexpression and activation is an early phenomenon caused by high-calorie/high-fat diet consumption and a likely contributor to kidney disease associated with prediabetes and alimentary obesity. |
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Authors:
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Hanna Shevalye; Sergey Lupachyk; Pierre Watcho; Roman Stavniichuk; Khaled Khazim; Hanna E Abboud; Irina G Obrosova |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-01-10 |
Journal Detail:
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Title: Endocrinology Volume: 153 ISSN: 1945-7170 ISO Abbreviation: Endocrinology Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-02-22 Completed Date: 2012-04-16 Revised Date: 2012-05-15 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 1152-61 Citation Subset: AIM; IM |
Affiliation:
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Pennington Biomedical Research Center, Louisiana State University, 6400 Perkins Road, Baton Rouge, Louisiana 70808, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed Animals Arachidonate 12-Lipoxygenase / metabolism Arachidonate 15-Lipoxygenase / metabolism Diabetic Nephropathies / diagnosis, etiology* Diet Diet, High-Fat / adverse effects* Kidney Cortex / metabolism Male Mice Mice, Inbred C57BL Models, Biological Obesity / metabolism Oxidative Stress* Podocytes / metabolism Prediabetic State / diagnosis, etiology Transforming Growth Factor beta / metabolism Transforming Growth Factor beta1 / metabolism Vascular Endothelial Growth Factor A / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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1P30-DK072476/DK/NIDDK NIH HHS; P20 RR021945/RR/NCRR NIH HHS; R01 DK078971/DK/NIDDK NIH HHS; R01DK074517/DK/NIDDK NIH HHS; R01DK077141/DK/NIDDK NIH HHS; R01DK078971/DK/NIDDK NIH HHS; R01DK081147/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/12-15-lipoxygenase; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 0/Vascular Endothelial Growth Factor A; EC 1.13.11.31/Arachidonate 12-Lipoxygenase; EC 1.13.11.33/Arachidonate 15-Lipoxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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