Document Detail

Precursor proteins are intermediates in vivo in the synthesis of two major outer membrane proteins, the OmpA and OmpF proteins, of Escherichia coli K12.
MedLine Citation:
PMID:  7009158     Owner:  NLM     Status:  MEDLINE    
The OmpA and OmpF proteins are major outer membrane proteins of Escherichia coli K12. Their precursors, the pro-OmpA and pro-OmpF proteins, have been detected in vivo in pulse-labelling experiments carried out with [35S]methionine at 25 degrees C. Wehn the pulse was at 37 degrees C, however, no precursors were detected. The pulse-labelled precursors were processed rapidly and quantitatively into mature protein at 25 degrees C. The apparent half-life of the pro-OmpF protein was estimated to be 30 s, and the pro-OmpA protein may be processed even faster. In short pulses (10 s) the precursors of both proteins were the predominant labelled species, indicating that at 25 degrees C processing does not start until chain elongation of the precursor is almost, if not entirely, complete. When French press lysates of cells pulse-labelled for 10 s were subjected to sucrose gradient centrifugation to separate the inner and outer membranes, both precursors comigrated with the inner membrane.
I Crowlesmith; K Gamon; U Henning
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  113     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1981 Jan 
Date Detail:
Created Date:  1981-05-28     Completed Date:  1981-05-28     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  375-80     Citation Subset:  IM    
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MeSH Terms
Centrifugation, Density Gradient
Electrophoresis, Polyacrylamide Gel
Escherichia coli / metabolism*
Membrane Proteins / biosynthesis*
Precipitin Tests
Protein Precursors / isolation & purification*
Reg. No./Substance:
0/Membrane Proteins; 0/Protein Precursors

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