Document Detail


Preconditioning somatothermal stimulation on right seventh intercostal nerve territory increases hepatic heat shock protein 70 and protects the liver from ischemia-reperfusion injury in rats.
MedLine Citation:
PMID:  11469906     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hyperthermic preconditioning attenuates the heat-induced cellular response to a subsequent severe heat challenge. However, it is impractical to perform whole-body hyperthermia in humans. This study was designed to test the hypotheses that hepatic heat shock protein 70 (Hsp70) could be induced by local somatothermal stimulation (LSTS) on right seventh intercostal nerve territory and that preconditioning the rats with LSTS protects the liver from subsequent ischemia-reperfusion injury. LSTS was brought about by application of a heating rod above right seventh intercostal nerve territory in male Sprague-Dawley rats. Hepatic gene expression of Hsp70 was assessed by Western blot and reverse transcription polymerase chain reaction (RT-PCR). Finally, serum ALT and AST and the lipid peroxidation product malondialdehyde (MDA) were evaluated in ischemic-reperfused rats preconditioned by application of LSTS on right seventh intercostal nerve territory. The results showed that hepatic gene expression of Hsp70 was upregulated in rats treated with LSTS. When animals were preconditioned with LSTS, followed by subsequent ischemia-reperfusion injury of the liver, there were significant decreases in liver enzymes (ALT/AST) and MDA formation in rats pretreated with one dose of LSTS (LSTS-1 group) as compared with those not treated with LSTS (control group) or treated with three doses of LSTS (LSTS-3 group). We conclude that mild local heat stress (one dose) on right seventh intercostal nerve territory upregulates hepatic gene expression of Hsp70 and protects the liver from subsequent ischemia-reperfusion injury. This might provide an easily applicable method for those patients facing ischemia-reperfusion challenge of the liver, as in liver resection and liver transplantation.
Authors:
Y H Lin; J H Chiu; H H Tung; M T Tsou; W Y Lui; C W Wu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of surgical research     Volume:  99     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-07-25     Completed Date:  2001-08-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  328-34     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Affiliation:
Department of Life Science, National Yang-Ming University, Li-Nong Street, Peitou, Taipei, 112, Taiwan, Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Gene Expression
HSP70 Heat-Shock Proteins / genetics*
Hot Temperature
Intercostal Nerves / physiology*
Ischemic Preconditioning*
Lipid Peroxidation / physiology
Liver / blood supply,  metabolism*
Male
Malondialdehyde / metabolism
Moxibustion
RNA, Messenger / analysis
Rats
Rats, Sprague-Dawley
Reperfusion Injury / metabolism*
Chemical
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 0/RNA, Messenger; 542-78-9/Malondialdehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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