Document Detail

Preconditioning effects on expression of proto-oncogenes c-fos and c-jun after hepatic ischemia/reperfusion in rats.
MedLine Citation:
PMID:  15908315     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Ischemia/reperfusion is the main cause of hepatic damage in liver transplantation. Immediate early genes (IEGs) encode proteins can regulate expression of cellular response genes after injury, and is associated with tissue repair and cell apoptosis. The purpose of this research was to investigate the effects of preconditioning on expression of immediate early genes c-fos and c-jun following hepatic ischemia/reperfusion (IR) and its roles in cellular regeneration and apoptosis. METHODS: Ninety-six Wistar rats were randomly divided into IR group and hepatic ischemic preconditioning (IPC) group, and each group was further divided into eight sub-groups (n=6). The model of partial liver ischemia/reperfusion was used. The rats were subjected to 60-minute liver ischemia, preceded by 10-minute preconditioning. After 0-, 0.5-, 1-, 2-, 4-, 8-, 12-, 24-hour reperfusion, the serum and liver tissue in each group were collected to detect the level of serum ALT/AST, liver histopathology, expression of c-fos, and c-jun mRNA. Flow cytometer was used to detect Ki67 and Sub-G1 as the quantity indicators of cell regeneration and apoptosis respectively. RESULTS: Compared with IR group, IPC group showed a significantly lower ALT/AST level in 0.5-hour sub-group to 8-hour sub-group (P<0.05). Ki67 elevated significantly at 0.5, 1, 2 hours, but decreased significantly at 24 hours (P<0.05). Ap index decreased significantly after 1-hour reperfusion (P<0.05). Expressions of c-fos and c-jun mRNA were low, especially c-jun at 0.5, 1 and 2 hours after reperfusion. CONCLUSION: Ischemic preconditioning can protect liver cells against ischemia/reperfusion injury, and this protective effect may be related to influence transcription levels of c-fos and c-jun.
Jian-Sheng Xiao; Fang-Gang Cai; Ying Niu; Yi Zhang; Xian-Ling Xu; Qi-Fa Ye
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hepatobiliary & pancreatic diseases international : HBPD INT     Volume:  4     ISSN:  1499-3872     ISO Abbreviation:  HBPD INT     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-23     Completed Date:  2005-07-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101151457     Medline TA:  Hepatobiliary Pancreat Dis Int     Country:  China    
Other Details:
Languages:  eng     Pagination:  197-202     Citation Subset:  IM    
Research Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
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MeSH Terms
Base Sequence
Biological Markers / analysis
Biopsy, Needle
Disease Models, Animal
Flow Cytometry
Ischemia / blood*,  surgery
Ischemic Preconditioning / methods*
Liver / blood supply*
Liver Function Tests
Molecular Sequence Data
Proto-Oncogene Proteins c-fos / analysis,  metabolism*
Proto-Oncogene Proteins c-jun / analysis,  metabolism*
RNA, Messenger / analysis
Random Allocation
Rats, Wistar
Reperfusion Injury / prevention & control*
Reverse Transcriptase Polymerase Chain Reaction
Risk Factors
Sensitivity and Specificity
Reg. No./Substance:
0/Biological Markers; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger

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