| Precocious terminal differentiation of premigratory limb muscle precursor cells requires positive signalling. | |
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MedLine Citation:
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PMID: 14991714 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The timing of myogenic differentiation of hypaxial muscle precursor cells in the somite lags behind that of epaxial precursors. Two hypotheses have been proposed to explain this delay. One attributes the delay to the presence of negative-acting signals from the lateral plate mesoderm adjacent to the hypaxial muscle precursor cells located in the ventrolateral lip of the somitic dermomyotome (Pourquié et al. [1995] Proc. Natl. Acad. Sci. USA 92:3219-3223). The second attributes the delay to an absence of positive-acting inductive signals, similar to those from the axial structures that induce epaxial myotome development (Pownall et al. [1996] Development 122:1475-1488). Because both studies relied principally upon changes in the expression pattern of mRNAs specific to early muscle precursor cell markers, we revisited these experiments using two methods to assess muscle terminal differentiation. First, injection of fluorescent dyes before surgery was used to determine whether ventrolateral lip cells transform from epithelial cells to elongated myocytes. Second, an antibody to a terminal differentiation marker and a new monoclonal antibody that recognises avian and mammalian Pax3 were used for immunohistochemistry to assess the transition from precursor cell to myocyte. The results support both hypotheses and show further that placing axial structures adjacent to the somite ventrolateral lip induces an axial pattern of myocyte terminal differentiation and elongation. |
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Authors:
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Sara J Venters; Rebecca E Argent; Fiona M Deegan; Gina Perez-Baron; Ted S Wong; William E Tidyman; Wilfred F Denetclaw; Christophe Marcelle; Marianne Bronner-Fraser; Charles P Ordahl |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: 229 ISSN: 1058-8388 ISO Abbreviation: Dev. Dyn. Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-03-01 Completed Date: 2004-10-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: United States |
Other Details:
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Languages: eng Pagination: 591-9 Citation Subset: IM; S |
Copyright Information:
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Copyright 2004 Wiley-Liss, Inc. |
Affiliation:
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Department of Anatomy, University of California, San Francisco, California 94143, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation Cell Movement Chick Embryo Coturnix DNA-Binding Proteins / metabolism Extremities / embryology* Fluorescent Dyes / pharmacology Immunohistochemistry In Situ Hybridization Microscopy, Fluorescence Models, Biological Muscles / cytology, embryology* MyoD Protein / metabolism Paired Box Transcription Factors RNA, Messenger / metabolism Signal Transduction Time Factors Transcription Factors / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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NS41070/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Fluorescent Dyes; 0/MyoD Protein; 0/Paired Box Transcription Factors; 0/RNA, Messenger; 0/Transcription Factors; 138016-91-8/Pax3 protein, mouse |
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