| Preclinical study of using multiphoton microscopy to diagnose liver cancer and differentiate benign and malignant liver lesions. | |
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MedLine Citation:
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PMID: 22463036 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Recently, the miniaturized multiphoton microscopy (MPM) and multiphoton probe allow the clinical use of multiphoton endoscopy for diagnosing cancer via "optical biopsy". The purpose of this study was to establish MPM optical diagnostic features for liver cancer and evaluate the sensitivity, specificity, and accuracy of MPM optical diagnosis. Firstly, we performed a pilot study to establish the MPM diagnostic features by investigating 60 surgical specimens, and found that high-resolution MPM images clearly demonstrated apparent differences between benign and malignant liver lesions in terms of their tissue architecture and cell morphology. Cancer cells, characterized by irregular size and shape, enlarged nuclei, and increased nuclear-cytoplasmic ratio, were identified by MPM images, which were comparable to hematoxylin-eosin staining images. Secondly, we performed a blinded study to evaluate the sensitivity, specificity, and accuracy of MPM optical diagnosis by investigating another 164 specimens, and found that the sensitivity, specificity, and accuracy of MPM diagnosis was 96.32%, 96.43%, and 96.34%, respectively. In conclusion, it is feasible to use MPM to diagnose liver cancer and differentiate benign and malignant liver lesions. This preclinical study provides the groundwork for further using multiphoton endoscopy to perform real-time noninvasive "optical biopsy" for liver lesions in the near future. |
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Authors:
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Jun Yan; Shuangmu Zhuo; Gang Chen; Xiufeng Wu; Dong Zhou; Shusen Xie; Jiahao Jiang; Mingang Ying; Fan Jia; Jianxin Chen; Jian Zhou |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biomedical optics Volume: 17 ISSN: 1560-2281 ISO Abbreviation: J Biomed Opt Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-04-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9605853 Medline TA: J Biomed Opt Country: United States |
Other Details:
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Languages: eng Pagination: 026004 Citation Subset: IM |
Affiliation:
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Fudan University, Zhongshan Hospital, Liver Cancer Institute, Shanghai 200032, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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