Document Detail

Preclinical evaluation of radiosensitizing activity of Pluronic block copolymers.
MedLine Citation:
PMID:  23631609     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Pluronic block copolymers are non-ionic surfactants with demonstrated sensitizing activity in chemotherapy and hyperthermia in various tumor cell lines. In the current study we investigated the potential activity of Pluronic as a radiosensitizing agent.
MATERIALS AND METHODS: As a possible mechanism, the effect of Pluronic on Hsp70 and Hsp90 was examined. Gli36 human glioma cells were treated with radiation alone as well as with a combination treatment of Pluronic and radiation.
RESULTS: Clonogenic cell survival assays show that Pluronic has an elevated effect on radiosensitization (50% high, p < 0.01), even with radiation doses as low as 2 Gy. The Hsp90 level was reduced 24 h after the combined treatment in both in vitro and in vivo. Similarly, Hsp70 levels were also decreased 24 h post treatment. When Gli36 cells were exposed to Pluronic before and during irradiation, DNA DSB: double-strand breaks repair was reduced, and elevated apoptosis was also seen in tumor xenografts.
CONCLUSION: Data suggest the potential use of L10 as a radiosensitizer. While the mechanism of sensitization requires additional investigation, the presented results indicate that the effect may be due, in part, to a decrease in Hsp90 and 70 levels and increased DNA damage.
Reshani H Perera; Ravi Patel; Hanping Wu; Mihika Gangolli; Bryan Traughber; Nancy Oleinick; Agata A Exner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-06-03
Journal Detail:
Title:  International journal of radiation biology     Volume:  89     ISSN:  1362-3095     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-10-02     Completed Date:  2013-11-19     Revised Date:  2014-10-09    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  801-12     Citation Subset:  IM; S    
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MeSH Terms
Apoptosis / drug effects,  radiation effects
Cell Line, Tumor
Cell Survival / drug effects,  radiation effects
HSP70 Heat-Shock Proteins / metabolism
HSP90 Heat-Shock Proteins / metabolism
Poloxamer / chemistry*,  pharmacology*
Radiation-Sensitizing Agents / chemistry*,  pharmacology*
Stem Cells / drug effects,  radiation effects
Xenograft Model Antitumor Assays
Grant Support
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 0/HSP90 Heat-Shock Proteins; 0/Radiation-Sensitizing Agents; 106392-12-5/Poloxamer

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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