Document Detail


Precision and long-term stability of capillary electrophoresis measurement of serum protein zones.
MedLine Citation:
PMID:  12809733     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Analytical evaluations of an available system for capillary zone electrophoresis (CZE) of serum protein have been reported. However, data concerning long-term precision and stability of the system, operated under routine conditions, are lacking. We report data from an internal quality control (QC) scheme, obtained over a 1-year period. METHODS: Measurements were done with a pair of instruments (Beckman Paragon CZE 2000 system), each equipped with seven capillaries. After preliminary (1 month) assessment of possible inter-capillary and inter-instrument variations, the QC material (a home prepared serum pool stored in the frozen state) was assayed daily over a 1-year period. RESULTS: Maximum inter-capillary and inter-instrument differences were 3.1% and 2.4%. No significant trend was observed for daily values (205 measurements over 1 year); in the same period overall imprecision values (CV) were in the interval 1.2% (albumin) to 3.2-6.1% (globulin zones). Mean monthly imprecision (CV) values were in the interval 1.1% (albumin) to 5.2% (globulin zones). There was no significant trend of monthly means with time. The observed imprecision values were within the biological variation-derived goals for imprecision. CONCLUSIONS: It is concluded that the assessed analytical instruments, operated in routine conditions, show long-term stability and imprecision consistent with the clinical use of the results produced.
Authors:
Paola Luraschi; Simona Brambilla; Ilenia Infusino; Carlo Franzini
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  333     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-17     Completed Date:  2004-02-10     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  41-5     Citation Subset:  IM    
Affiliation:
Laboratorio di Chimica Clinica, Ospedale L. Sacco, Milan, Italy.
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MeSH Terms
Descriptor/Qualifier:
Blood Proteins / analysis*
Electrophoresis, Capillary / instrumentation,  methods*
Humans
Quality Control
Reference Values
Reproducibility of Results
Chemical
Reg. No./Substance:
0/Blood Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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