Document Detail

Precise genome modification in the crop species Zea mays using zinc-finger nucleases.
MedLine Citation:
PMID:  19404259     Owner:  NLM     Status:  MEDLINE    
Agricultural biotechnology is limited by the inefficiencies of conventional random mutagenesis and transgenesis. Because targeted genome modification in plants has been intractable, plant trait engineering remains a laborious, time-consuming and unpredictable undertaking. Here we report a broadly applicable, versatile solution to this problem: the use of designed zinc-finger nucleases (ZFNs) that induce a double-stranded break at their target locus. We describe the use of ZFNs to modify endogenous loci in plants of the crop species Zea mays. We show that simultaneous expression of ZFNs and delivery of a simple heterologous donor molecule leads to precise targeted addition of an herbicide-tolerance gene at the intended locus in a significant number of isolated events. ZFN-modified maize plants faithfully transmit these genetic changes to the next generation. Insertional disruption of one target locus, IPK1, results in both herbicide tolerance and the expected alteration of the inositol phosphate profile in developing seeds. ZFNs can be used in any plant species amenable to DNA delivery; our results therefore establish a new strategy for plant genetic manipulation in basic science and agricultural applications.
Vipula K Shukla; Yannick Doyon; Jeffrey C Miller; Russell C DeKelver; Erica A Moehle; Sarah E Worden; Jon C Mitchell; Nicole L Arnold; Sunita Gopalan; Xiangdong Meng; Vivian M Choi; Jeremy M Rock; Ying-Ying Wu; George E Katibah; Gao Zhifang; David McCaskill; Matthew A Simpson; Beth Blakeslee; Scott A Greenwalt; Holly J Butler; Sarah J Hinkley; Lei Zhang; Edward J Rebar; Philip D Gregory; Fyodor D Urnov
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Publication Detail:
Type:  Journal Article     Date:  2009-04-29
Journal Detail:
Title:  Nature     Volume:  459     ISSN:  1476-4687     ISO Abbreviation:  Nature     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-21     Completed Date:  2009-07-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0410462     Medline TA:  Nature     Country:  England    
Other Details:
Languages:  eng     Pagination:  437-41     Citation Subset:  IM    
Dow AgroSciences, 9330 Zionsville Road, Indianapolis, Indiana 46268, USA.
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MeSH Terms
Biotechnology / methods*
Deoxyribonucleases / chemistry*,  genetics,  metabolism*
Food, Genetically Modified
Gene Targeting / methods*
Genes, Plant / genetics
Genome, Plant / genetics*
Herbicide Resistance / genetics
Herbicides / pharmacology
Inositol Phosphates / metabolism
Mutagenesis, Site-Directed / methods
Plants, Genetically Modified
Recombination, Genetic / genetics
Reproducibility of Results
Zea mays / genetics*
Zinc Fingers*
Reg. No./Substance:
0/Herbicides; 0/Inositol Phosphates; EC 3.1.-/Deoxyribonucleases
Comment In:
Nature. 2009 May 21;459(7245):337-8   [PMID:  19458706 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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