| Prebiotic evaluation of a novel galactooligosaccharide mixture produced by the enzymatic activity of Bifidobacterium bifidum NCIMB 41171, in healthy humans: a randomized, double-blind, crossover, placebo-controlled intervention study. | |
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MedLine Citation:
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PMID: 18326619 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Galactooligosaccharides are selectively fermented by the beneficial member of the colonic microflora contributing to the health of the host. OBJECTIVE: We assessed the prebiotic potential of a novel galactooligosaccharide produced through the action of beta-galactosidases, originating from a probiotic Bifidobacterium bifidum strain, against a galactooligosaccharide produced through the action of an industrial beta-galactosidase and a placebo. DESIGN: Fifty-nine healthy human volunteers participated in this study. Initially, the effect of the matrix on the prebiotic properties of a commercially available galactooligosaccharide (7 g/d) was assessed during 7-d treatment periods with a 7-d washout period in between. During the second phase, 30 volunteers were assigned to a sequence of treatments (7 d) differing in the amount of the novel galactooligosaccharide (0, 3.6, or 7 g/d). Stools were recovered before and after each intervention, and bacteria numbers were determined by fluorescent in situ hybridization. RESULTS: Addition of the novel galactooligosaccharide mixture significantly increased the bifidobacterial population ratio compared with the placebo (P < 0.05), whereas 7 g/d of the novel galactooligosaccharide significantly increased the bifidobacterial ratio compared with the commercial galactooligosaccharide (P < 0.05). Moreover, a significant relation (P < 0.001) between the bifidobacteria proportion and the novel galactooligosaccharide dose (0, 3.6, and 7 g/d) was observed. This relation was similar to the effect of the novel galactooligosaccharide on the prebiotic index of each dose. CONCLUSIONS: This study showed that galactooligosaccharide mixtures produced with different beta-galactosidases show different prebiotic properties and that, by using enzymes originating from bifidobacterial species, an increase in the bifidogenic properties of the prebiotic product is achievable. |
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Authors:
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Flore Depeint; George Tzortzis; Jelena Vulevic; Kerry I'anson; Glenn R Gibson |
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Publication Detail:
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Type: Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of clinical nutrition Volume: 87 ISSN: 1938-3207 ISO Abbreviation: Am. J. Clin. Nutr. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-10 Completed Date: 2008-04-15 Revised Date: 2009-05-15 |
Medline Journal Info:
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Nlm Unique ID: 0376027 Medline TA: Am J Clin Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 785-91 Citation Subset: AIM; IM |
Affiliation:
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School of Food Biosciences, The University of Reading, Reading, United Kingdom, and the Institute of Food Research, Colney, Norwich, United Kingdom. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Bifidobacterium / enzymology* Colon / metabolism, microbiology Cross-Over Studies Dose-Response Relationship, Drug Double-Blind Method Feces / microbiology Female Fermentation Galactose / biosynthesis, metabolism* Humans In Situ Hybridization, Fluorescence / methods Male Oligosaccharides / biosynthesis, metabolism* Probiotics* beta-Galactosidase / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Oligosaccharides; 26566-61-0/Galactose; EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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