| Preanalytical variables in measurement of free (ionized) calcium in lithium heparin-containing blood collection tubes. | |
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MedLine Citation:
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PMID: 19361462 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Measurements of free (ionized) calcium (iCa) are increasingly requested in patient care locations where immediate analysis is unavailable. Evacuated blood collection tubes containing lithium heparin and gel separator material are widely used in clinical laboratories, but little information is available on the effects of these tubes or of delay prior to analysis on the concentration or stability of iCa. METHODS: We collected blood from volunteers into lithium-heparin tubes (PST, Vacutainer PST, BD Pre-Analytic Systems) of multiple lots and into electrolyte-balanced heparin syringes (Portex Dry Heparin, Smiths Medical). iCa was measured (Siemens 1265 blood gas analyzers) immediately and, in PST, at 0-7 h with or without transportation of the tubes from remote sites. RESULTS: The mean difference of free calcium results in the PST tubes and electrolyte-balanced syringes was -0.08 (95% confidence interval -0.17 to 0.012) mmol/l, and the SD of the residuals (Sy, x) of the regression was 0.03 mmol/l. There was no detectable lot-to-lot variation in results. Free calcium was stable in tubes at room temperature and at 4 degrees C for at least 7 h with or without transportation. CONCLUSION: iCa measured in the examined blood collection tubes is stable and unaffected by lot-to-lot variation of tubes, but results are slightly lower than with special blood gas syringes. |
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Authors:
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Doris M Haverstick; Louis B Brill; Mitchell G Scott; David E Bruns |
Publication Detail:
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Type: Journal Article Date: 2009-02-04 |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 403 ISSN: 1873-3492 ISO Abbreviation: Clin. Chim. Acta Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-27 Completed Date: 2009-07-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 102-4 Citation Subset: IM |
Affiliation:
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Department of Pathology, University of Virginia, Charlottesville, VA, United States. dmh2t@virginia.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blood Specimen Collection
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methods* Calcium / blood* Heparin* Humans Laboratories Lithium Temperature Time Factors |
| Chemical | |
Reg. No./Substance:
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7439-93-2/Lithium; 7440-70-2/Calcium; 9005-49-6/Heparin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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