| Preanalytical stability of HIV-1 and HCV RNA: impact of storage and plasma separation from cells on blood donation testing by NAT. | |
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MedLine Citation:
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PMID: 21092012 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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BACKGROUND: The aim of this study was to evaluate the optimal preanalytical conditions prior to nucleic acid amplification technology (NAT) for human immunodeficiency virus-1 (HIV-1) or Hepatitis C virus (HCV) RNA in pools of 96 plasma specimens with regard to storage temperature, time and plasma separation in a blood donation environment. STUDY DESIGN AND METHODS: Changes in viral nucleic acid concentration of HIV-1 and HCV were observed for 5 days according to the Paul-Ehrlich-Institute's (PEI) guidelines that demand 95%-detection limit of at least 10 000 IU mL(-1) for HIV-1 RNA and 5000 IU mL(-1) for HCV RNA within a single donor blood specimen. Ninety-five per cent detection limits of HIV-1 RNA over 3 days after storage at either 5 or 21 °C were evaluated by using standardised HIV-1 RNA-positive plasma. RESULTS: HCV RNA in whole blood samples proved to be more stable than HIV-1 RNA. Whole blood storage at 21 °C was shown to decrease the detectability of HIV-1 RNA even after only 18 h. Plasma samples once used for NAT at time 18 h did not alter viral stability up to 48 h after donation. Ninety-five per cent detection limits of HIV-1 RNA were securely below 10 000 IU mL(-1) for 24 h after whole blood storage at 5 °C. CONCLUSIONS: These results may lead to a discussion around the most suitable preanalytical conditions in blood donation environments. Contrary to the current PEI guidelines that allow storage of whole blood specimens up to 18 h at 21 °C, these results suggest that immediate storage in a 5 °C container after blood donation is more suitable and would permit storage of whole blood up to 24 h prior to the separation of plasma from cells. |
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Authors:
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T J Schulze; C Weiss; J Luhm; C Brockmann; S Görg; H Hennig |
Publication Detail:
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Type: Journal Article Date: 2010-11-23 |
Journal Detail:
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Title: Transfusion medicine (Oxford, England) Volume: 21 ISSN: 1365-3148 ISO Abbreviation: Transfus Med Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-02-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9301182 Medline TA: Transfus Med Country: England |
Other Details:
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Languages: eng Pagination: 99-106 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Transfusion Medicine © 2010 British Blood Transfusion Society. |
Affiliation:
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Institute of Transfusion Medicine and Immunlogie, German Red Cross Blood Service, Baden Württemberg-Hessen, Medical Faculty of Mannheim, Heidelberg University, Mannheim, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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