| A Pre-targeting Strategy for MR Imaging of Functional Molecules Using Dendritic Gd-Based Contrast Agents. | |
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MedLine Citation:
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PMID: 21140232 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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PURPOSE: We aimed to establish a magnetic resonance imaging (MRI) protocol for the sensitive and specific imaging of functional molecules with a pre-targeting strategy utilizing the streptavidin-biotin interaction. Membrane type-1 matrix metalloproteinase (MT1-MMP) was selected as the target molecule. PROCEDURES: The biotinylated polyamidoamine dendrimer (PAMAM)-based contrast agent (Bt-PAMAM-DTPA(Gd)) was prepared, and its proton relaxivity (r1) and affinity to streptavidin were evaluated. Tumor-bearing mice were pre-targeted with streptavidin-conjugated anti-MT1-MMP monoclonal antibody (mAb), streptavidin-conjugated negative control IgG, or saline and 3 days later were injected with Bt-PAMAM-DTPA(Gd) followed immediately by MRI for a period of 3 h. RESULTS: High r1 (15.5 L mmol(-1) s(-1)) and 1.9-fold higher affinity than D: -biotin were obtained. Significantly higher relative tumor signals were observed in mice pre-targeted with streptavidin-conjugated anti-MT1-MMP mAb (165% at 3 h vs. pre-administration) than with saline or streptavidin-conjugated negative control IgG (P < 0.0001). CONCLUSIONS: This pre-targeting approach can accomplish sensitive and specific in vivo MRI of functional molecules. |
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Authors:
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Kohei Sano; Takashi Temma; Takashi Azuma; Ryusuke Nakai; Michiko Narazaki; Yuji Kuge; Hideo Saji |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging Volume: 13 ISSN: 1860-2002 ISO Abbreviation: Mol Imaging Biol Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-11-09 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101125610 Medline TA: Mol Imaging Biol Country: United States |
Other Details:
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Languages: eng Pagination: 1196-203 Citation Subset: IM |
Affiliation:
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Department of Patho-Functional Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto, 606-8501, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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