Document Detail

Pre- and postimplantation development of swine-cloned embryos derived from fibroblasts and bone marrow cells after inhibition of histone deacetylases.
MedLine Citation:
PMID:  20132016     Owner:  NLM     Status:  MEDLINE    
The present study assessed changes in epigenetic markers and pre- and postimplantation development in somatic cell nuclear transfer (SCNT) porcine embryos after treatment with the inhibitor of histone deacetylases (HDACi), Trichostatin A (TSA). Embryos were generated using in vitro matured oocytes and nuclei from either a male fetal fibroblast (FF) cell line or bone marrow cells (BMC) from three adult sows. After nuclear transfer, oocytes were either exposed or not to 10 ng/mL TSA for 10 h starting 1 h after cell fusion. Samples of one-cell stage and cleaved (two- to four-cell stage) embryos were fixed at 15 to 18 h or 46 to 48 h after cell fusion and immunocytochemically processed to detect histone H3 acetylation at lysine 14 (H3K14ac) or histone H3 dimethylation at lysine 9 (H3K9m2) using specific primary antibodies. TSA treatment increased the immunofluorescent signal for H3K14ac in cleaved embryos derived from both FF and BMC but did not affect H3K9m2. Development to the blastocyst stage was increased by TSA treatment (45.2 vs. 23.9%) in embryos produced from FF cells but not in those produced from BMC (30.6 vs. 27.4%). Cloned piglets were produced from both treatments when day 5 to 6 blastocyst-stage embryos derived from FF cells were transferred into the uterus of recipient females. Cloned piglets were also produced after the transfer of TSA-treated blastocysts derived from BMC of adult sows but not from control embryos. These findings suggest that the inhibition of histone deacetylases have similar effects on enhancing H3K14ac in SCNT embryos reconstructed from different cell types but the effect on in vitro and in vivo development seems to differ according to the nuclear donor cell type.
Mario A Martinez-Diaz; Limei Che; Marcelo Albornoz; Marcelo M Seneda; Daryn Collis; Ana Rita S Coutinho; Nayala El-Beirouthi; Denyse Laurin; Xin Zhao; Vilceu Bordignon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular reprogramming     Volume:  12     ISSN:  2152-4998     ISO Abbreviation:  Cell Reprogram     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-05     Completed Date:  2010-05-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101528176     Medline TA:  Cell Reprogram     Country:  United States    
Other Details:
Languages:  eng     Pagination:  85-94     Citation Subset:  IM    
Department of Animal Science, McGill University, Ste-Anne-de-Bellevue, Qu?bec, Canada.
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MeSH Terms
Blastocyst / drug effects*,  metabolism,  physiology
Bone Marrow Cells / metabolism
Cell Nucleus / metabolism
Cells, Cultured
Cloning, Organism / methods
Embryo Culture Techniques
Embryo Transfer
Embryo, Mammalian / drug effects,  metabolism
Embryonic Development / drug effects*
Fibroblasts / metabolism
Histone Deacetylase Inhibitors / pharmacology
Histone Deacetylases / metabolism*
Hydroxamic Acids / pharmacology
Nuclear Transfer Techniques / veterinary*
Oocytes / metabolism
Time Factors
Reg. No./Substance:
0/Histone Deacetylase Inhibitors; 0/Hydroxamic Acids; 58880-19-6/trichostatin A; EC Deacetylases

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