Document Detail

Pre-column derivatization and its optimum conditions for quantitative determination of urinary hydroxylysine glycosides by high-performance liquid chromatography.
MedLine Citation:
PMID:  8364515     Owner:  NLM     Status:  MEDLINE    
A method has been developed for rapid analysis of the urinary hydroxylysine glycosides [glucosyl-galactosyl-hydroxylysine (GGH) and galactosyl-hydroxylysine (GH)] as collagen metabolites. The glycosides were fluorometrically derivatized with 5-dimethylaminonaphthalene-1-sulfonyl chloride, and then subjected to reversed-phase high-performance liquid chromatography using a gradient system. Suitable standard hydroxylysine glycosides which were not diastereoisomers were prepared from human urine. Urinary GGH and GH were analyzed within 1 h, including column washing. This method was applied to the urine samples of 68 healthy persons of different ages.
K Yoshihara; N Mochidome; Y Shida; Y Hayakawa; M Nagata
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  16     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-10-04     Completed Date:  1993-10-04     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  604-7     Citation Subset:  IM    
Tokyo College of Pharmacy, Japan.
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MeSH Terms
Aging / metabolism
Chromatography, High Pressure Liquid
Dansyl Compounds / analysis,  chemical synthesis
Glycosides / urine
Hydroxylysine / urine*
Indicators and Reagents
Middle Aged
Spectrometry, Fluorescence
Reg. No./Substance:
0/Dansyl Compounds; 0/Glycosides; 0/Indicators and Reagents; 28902-93-4/Hydroxylysine

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