Document Detail


Practical structured illumination microscopy.
MedLine Citation:
PMID:  25391800     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Structured illumination microscopy (SIM) is a method that can double the spatial resolution of wide-field fluorescence microscopy in three dimensions by using spatially structured illumination light. In this chapter, we introduce the basic principles of SIM and describe in detail several different implementations based on either a diffraction grating or liquid crystal spatial light modulators. We also describe nonlinear SIM, a method that in theory can achieve unlimited resolution. In addition, we discuss a number of key points important for high-resolution imaging.
Authors:
E Hesper Rego; Lin Shao
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  1251     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2015  
Date Detail:
Created Date:  2014-11-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  175-92     Citation Subset:  IM    
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