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Practical recommendations for the reduction of memory effects in compound-specific (15) N/(14) N-ratio analysis of enriched amino acids by gas chromatography/combustion/isotope ratio mass spectrometry.
MedLine Citation:
PMID:  22173808     Owner:  NLM     Status:  In-Data-Review    
Gas chromatography/combustion/isotope ratio mass spectrometry (GC-C-IRMS) is a highly sensitive approach which allows the analysis of the (13) C/(12) C and (15) N/(14) N isotope composition of amino acids in the range of natural abundance or in slightly (13) C- and (15) N-enriched samples. However, the accuracy of measurements remains a permanent challenge. Here we show the effect of the presence of slightly (15) N-enriched compounds in physiological samples on the accuracy and reproducibility of (15) N-abundances of amino acids within or between analytical runs. We spiked several individual amino acids with the respective (15) N-labelled isotopomer and measured the (15) N/(14) N ratios of other amino acids in the same sample or in the following analytical runs. Intra- and inter-run memory effects can be observed in (15) N/(14) N ratios of amino acids. Sample throughput is reduced when cleaning runs using standard mixtures are required to restore initial conditions after runs of samples with (15) N-enriched analytes. Possible reasons for the observed phenomenon and its implications for work in the lower (15) N-enrichment range (<0.5 APE) are discussed and include different aspects of gas chromatography, derivatisation, and hot catalytic metal surface effects. Results need to be interpreted with caution if complex physiological samples contain (15) N-enriched amino acids beyond 500‰ δ(15) N (~0.18 APE). Copyright © 2011 John Wiley & Sons, Ltd.
Klaus J Petzke; Cornelia C Metges
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  26     ISSN:  1097-0231     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  195-204     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 John Wiley & Sons, Ltd.
German Institute of Human Nutrition in Potsdam-Rehbruecke (DIfE), Arthur-Scheunert-Allee 114-116, 14558, Nuthetal, Germany.
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