| Practical recommendations for the reduction of memory effects in compound-specific (15) N/(14) N-ratio analysis of enriched amino acids by gas chromatography/combustion/isotope ratio mass spectrometry. | |
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MedLine Citation:
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PMID: 22173808 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Gas chromatography/combustion/isotope ratio mass spectrometry (GC-C-IRMS) is a highly sensitive approach which allows the analysis of the (13) C/(12) C and (15) N/(14) N isotope composition of amino acids in the range of natural abundance or in slightly (13) C- and (15) N-enriched samples. However, the accuracy of measurements remains a permanent challenge. Here we show the effect of the presence of slightly (15) N-enriched compounds in physiological samples on the accuracy and reproducibility of (15) N-abundances of amino acids within or between analytical runs. We spiked several individual amino acids with the respective (15) N-labelled isotopomer and measured the (15) N/(14) N ratios of other amino acids in the same sample or in the following analytical runs. Intra- and inter-run memory effects can be observed in (15) N/(14) N ratios of amino acids. Sample throughput is reduced when cleaning runs using standard mixtures are required to restore initial conditions after runs of samples with (15) N-enriched analytes. Possible reasons for the observed phenomenon and its implications for work in the lower (15) N-enrichment range (<0.5 APE) are discussed and include different aspects of gas chromatography, derivatisation, and hot catalytic metal surface effects. Results need to be interpreted with caution if complex physiological samples contain (15) N-enriched amino acids beyond 500‰ δ(15) N (~0.18 APE). Copyright © 2011 John Wiley & Sons, Ltd. |
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Authors:
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Klaus J Petzke; Cornelia C Metges |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Rapid communications in mass spectrometry : RCM Volume: 26 ISSN: 1097-0231 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2011-12-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
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Languages: eng Pagination: 195-204 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 John Wiley & Sons, Ltd. |
Affiliation:
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German Institute of Human Nutrition in Potsdam-Rehbruecke (DIfE), Arthur-Scheunert-Allee 114-116, 14558, Nuthetal, Germany. petzke@dife.de. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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